Revealing a mechanistic understanding of the role of viruses and host nutrient status in modulating CO2 fixation in key marine phototrophs
揭示病毒和宿主营养状态在调节关键海洋光养生物二氧化碳固定中的作用的机制理解
基本信息
- 批准号:NE/N003241/1
- 负责人:
- 金额:$ 49.22万
- 依托单位:
- 依托单位国家:英国
- 项目类别:Research Grant
- 财政年份:2016
- 资助国家:英国
- 起止时间:2016 至 无数据
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
The oceans play a major role in determining world climate. In part, this is due to the production of oxygen and the consumption of carbon dioxide by very small, single celled organisms, which are referred to as the photosynthetic picoplankton. Marine cyanobacteria of the closely-related genera Prochlorococcus and Synechococcus are the prokaryotic components of the photosynthetic picoplankton. These cyanobacteria are continually growing and dividing, but they can also be infected and killed by viruses. Viruses that infect bacteria (bacteriophage) have provided the basis of our current understanding of molecular biology and genetics and have recently assumed a much greater significance with the recognition of the extraordinary abundance of bacteriophages and their central role in many biological processes. Cyanophages are viruses that are specifically capable of infecting a type of bacteria (cyanobacteria) that utilises light as its primary energy source through the process of photosynthesis. The cyanobacterial photosynthetic machinery captures light energy and transfers it to chemical energy which is subsequently used for growth and replication. Oceanic regions vary considerably in their supply of nutrients e.g. phosphate, nitrogen and iron, that are critical for the growth of cyanobacteria, potentially limiting CO2 fixation by these organisms. The availability of nutrients may also affect cyanophage replication, since during infection cyanophage rely on their hosts to provide them with enough energy and resources to allow them to replicate efficiently. However, the effect of nutrient availability on marine cyanobacterial CO2 fixation in the presence and absence of phage infection is largely unknown. This is important because marine cyanobacteria are critical contributors to global CO2 fixation and virus infection of these organisms may significantly modulate this contribution. One exception is that phosphate limitation of marine Synechococcus has been shown to cause an 80% reduction in the number of cyanophage produced with <10% of cells lysing. Cyanophage infect P starved cells but remain inside their hosts without killing them, in a state known as 'pseudolysogeny'. Given that oceanic systems are often depleted in nutrients such as P (as well as nitrogen (N) and iron (Fe)) suggests such infection dynamics are likely widely prevalent in the natural environment.Hence, in this proposal we will determine the role that nutrient limited growth plays on marine cyanobacteria CO2 fixation rates in the presence and absence of phage infection. We will also assess the role that specific cyanophage genes contribute to the process, and determine the molecular basis regulating 'pseudolysogeny'. Moreover, we will also provide a reliable (experimentally-derived) mathematical formulation describing viral infection which will be incorporated into an Ecosystem Model [ERSEM] providing a substantially improved simulation of oceanic primary production.Overall, the proposal will therefore provide direct estimates, and a mechanistic basis, for understanding the role of nutrients and cyanophage infection in controlling marine primary production. Data and concepts will subsequently be used in ERSEM to refine control points for marine photosynthesis and subsequent C cycling.
海洋在决定世界气候方面起着重要作用。在某种程度上,这是由于非常小的单细胞生物产生氧气和消耗二氧化碳,这些生物被称为光合作用的浮游生物。原绿球藻属和聚球藻属的海洋蓝藻是光合浮游生物的原核成分。这些蓝藻不断地生长和分裂,但它们也会被病毒感染和杀死。感染细菌的病毒(噬菌体)为我们目前对分子生物学和遗传学的理解提供了基础,最近随着人们认识到噬菌体的异常丰富及其在许多生物过程中的核心作用而具有更大的意义。噬藻病毒是一种能够感染一种细菌(蓝藻)的病毒,这种细菌通过光合作用将光作为主要能量来源。蓝藻的光合作用机制捕获光能并将其转化为化学能,随后用于生长和复制。海洋区域的营养物供应差异很大,如磷酸盐、氮和铁,这些营养物对蓝藻的生长至关重要,可能限制这些生物对二氧化碳的固定。营养物质的可用性也可能影响噬藻体的复制,因为在感染期间,噬藻体依赖宿主为它们提供足够的能量和资源,以使它们能够有效地复制。然而,在存在和不存在噬菌体感染的情况下,营养物有效性对海洋蓝藻CO2固定的影响在很大程度上是未知的。这一点很重要,因为海洋蓝藻是全球二氧化碳固定的关键贡献者,这些生物的病毒感染可能会显著调节这一贡献。一个例外是,海洋聚藻球菌的磷酸盐限制已被证明导致在细胞裂解率<10%的情况下产生的噬藻体数量减少80%。噬藻体会感染饥饿的P细胞,但会留在宿主体内而不会杀死它们,这种状态被称为“伪溶解”。鉴于海洋系统中磷(以及氮(N)和铁(Fe))等营养物质经常耗尽,这表明这种感染动态可能在自然环境中广泛存在。因此,在本提案中,我们将确定在存在和不存在噬菌体感染的情况下,营养限制生长对海洋蓝藻二氧化碳固定率的作用。我们还将评估特定的噬藻基因在这一过程中的作用,并确定调节“假溶解”的分子基础。此外,我们还将提供一个可靠的(实验推导的)描述病毒感染的数学公式,该公式将被纳入生态系统模型[ERSEM],从而大大改进海洋初级生产的模拟。总的来说,该提案将因此为了解营养物和噬藻体感染在控制海洋初级生产中的作用提供直接估计和机制基础。数据和概念随后将在ERSEM中使用,以细化海洋光合作用和随后的C循环的控制点。
项目成果
期刊论文数量(10)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Elucidating the picocyanobacteria salinity divide through ecogenomics of new freshwater isolates.
- DOI:10.1186/s12915-022-01379-z
- 发表时间:2022-08-08
- 期刊:
- 影响因子:5.4
- 作者:Cabello-Yeves, Pedro J.;Callieri, Cristiana;Picazo, Antonio;Schallenberg, Lena;Huber, Paula;Roda-Garcia, Juan J.;Bartosiewicz, Maciej;Belykh, Olga, I;Tikhonova, Irina, V;Torcello-Requena, Alberto;De Prado, Paula Martin;Puxty, Richard J.;Millard, Andrew D.;Camacho, Antonio;Rodriguez-Valera, Francisco;Scanlan, David J.
- 通讯作者:Scanlan, David J.
Relative stability of ploidy in a marine Synechococcus across various growth conditions.
海洋聚球藻倍性在各种生长条件下的相对稳定性。
- DOI:10.1111/1758-2229.12614
- 发表时间:2018
- 期刊:
- 影响因子:3.3
- 作者:Perez-Sepulveda B
- 通讯作者:Perez-Sepulveda B
Analysis of Selection Methods to Develop Novel Phage Therapy Cocktails Against Antimicrobial Resistant Clinical Isolates of Bacteria.
- DOI:10.3389/fmicb.2021.613529
- 发表时间:2021
- 期刊:
- 影响因子:5.2
- 作者:Haines MEK;Hodges FE;Nale JY;Mahony J;van Sinderen D;Kaczorowska J;Alrashid B;Akter M;Brown N;Sauvageau D;Sicheritz-Pontén T;Thanki AM;Millard AD;Galyov EE;Clokie MRJ
- 通讯作者:Clokie MRJ
Global Phylogeography of Marine Synechococcus in Coastal Areas Reveals Strong Community Shifts.
- DOI:10.1128/msystems.00656-22
- 发表时间:2022-12-20
- 期刊:
- 影响因子:6.4
- 作者:
- 通讯作者:
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
数据更新时间:{{ journalArticles.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ monograph.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ sciAawards.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ conferencePapers.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ patent.updateTime }}
David Scanlan其他文献
David Scanlan的其他文献
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
{{ truncateString('David Scanlan', 18)}}的其他基金
Why do alpha-cyanobacteria with form 1A RuBisCO dominate aquatic habitats worldwide? (CYANORUB)
为什么具有 1A 型 RuBisCO 的 α-蓝藻在全世界的水生栖息地中占主导地位?
- 批准号:
EP/Y028384/1 - 财政年份:2024
- 资助金额:
$ 49.22万 - 项目类别:
Fellowship
Elucidating the consequences of picocyanobacterial lipid remodelling for global marine primary production estimates
阐明微微蓝藻脂质重塑对全球海洋初级生产力估算的影响
- 批准号:
NE/V000373/1 - 财政年份:2021
- 资助金额:
$ 49.22万 - 项目类别:
Research Grant
JTS-100: A step change in accurately measuring photosynthesis
JTS-100:精确测量光合作用的重大变革
- 批准号:
NE/T008962/1 - 财政年份:2019
- 资助金额:
$ 49.22万 - 项目类别:
Research Grant
Protistan grazing and viral infection of marine picoplankton: a role for the host cell surface?
海洋超微型浮游生物的原生生物放牧和病毒感染:宿主细胞表面的作用?
- 批准号:
NE/J02273X/1 - 财政年份:2012
- 资助金额:
$ 49.22万 - 项目类别:
Research Grant
Elucidating niche adaptation mechanisms in a ubiquitous marine phototroph: a targeted 'omics approach
阐明普遍存在的海洋光养生物的生态位适应机制:有针对性的“组学方法”
- 批准号:
NE/I00985X/1 - 财政年份:2011
- 资助金额:
$ 49.22万 - 项目类别:
Research Grant
Regulatory gene networks and ecological distinctness in marine Synechococcus
海洋聚球藻的调控基因网络和生态独特性
- 批准号:
NE/G017948/1 - 财政年份:2010
- 资助金额:
$ 49.22万 - 项目类别:
Research Grant
How important is prokaryotic photoheterotrophy in ecosystems of the Atlantic Ocean from 40oS to 40oN?
原核光异养在南纬 40 度到北纬 40 度的大西洋生态系统中有多重要?
- 批准号:
NE/H007083/1 - 财政年份:2010
- 资助金额:
$ 49.22万 - 项目类别:
Research Grant
Dissecting, and revealing the controls on, the group-specific CO2 fixation budget of the Atlantic Ocean
剖析并揭示对大西洋特定群体二氧化碳固定预算的控制
- 批准号:
NE/G005125/1 - 财政年份:2009
- 资助金额:
$ 49.22万 - 项目类别:
Research Grant
Metal composition of marine cyanobacteria - an indicator of niche adaptation and cell physiological state?
海洋蓝藻的金属成分 - 生态位适应和细胞生理状态的指标?
- 批准号:
NE/F004249/1 - 财政年份:2008
- 资助金额:
$ 49.22万 - 项目类别:
Research Grant
Defining the molecular basis of phylogenetic diversity in marine Synechococcus / a genomic approach
定义海洋聚球藻系统发育多样性的分子基础/基因组方法
- 批准号:
NE/D003385/1 - 财政年份:2006
- 资助金额:
$ 49.22万 - 项目类别:
Research Grant
相似国自然基金
酶响应的中性粒细胞外泌体载药体系在眼眶骨缺损修复中的作用及机制研究
- 批准号:82371102
- 批准年份:2023
- 资助金额:49.00 万元
- 项目类别:面上项目
HIF-1α调控软骨细胞衰老在骨关节炎进展中的作用及机制研究
- 批准号:82371603
- 批准年份:2023
- 资助金额:49.00 万元
- 项目类别:面上项目
脐带间充质干细胞微囊联合低能量冲击波治疗神经损伤性ED的机制研究
- 批准号:82371631
- 批准年份:2023
- 资助金额:49.00 万元
- 项目类别:面上项目
TRIM25-PHGDH信号轴调控脓毒症肺上皮细胞铁死亡的机制研究
- 批准号:82372151
- 批准年份:2023
- 资助金额:48.00 万元
- 项目类别:面上项目
α-酮戊二酸调控ACMSD介导犬尿氨酸通路代谢重编程在年龄相关性听力损失中的作用及机制研究
- 批准号:82371150
- 批准年份:2023
- 资助金额:49.00 万元
- 项目类别:面上项目
芍药苷靶向α-烯醇化酶治疗实验性自身免疫性脑脊髓炎的机制研究
- 批准号:82371809
- 批准年份:2023
- 资助金额:49.00 万元
- 项目类别:面上项目
脂滴聚集型小胶质细胞介导的髓鞘病变促进小鼠抑郁样行为及其机制研究
- 批准号:82371528
- 批准年份:2023
- 资助金额:49.00 万元
- 项目类别:面上项目
基于AMPK/PGC-1α信号轴的工程化外泌体靶向调控BMSCs能量代谢重编程在老年机体骨修复中的作用及其机制研究
- 批准号:82370920
- 批准年份:2023
- 资助金额:48.00 万元
- 项目类别:面上项目
星形胶质细胞介导的髓鞘吞噬参与慢性脑低灌注白质损伤的机制研究
- 批准号:82371307
- 批准年份:2023
- 资助金额:49.00 万元
- 项目类别:面上项目
相似海外基金
Collaborative Research: Mechanistic understanding of chemomechanics in phase-changing electroceramics for sodium-ion batteries
合作研究:钠离子电池相变电陶瓷化学力学的机理理解
- 批准号:
2325464 - 财政年份:2024
- 资助金额:
$ 49.22万 - 项目类别:
Continuing Grant
Extreme Climatic Events in the Oceans: Towards a mechanistic understanding of ecosystem impacts and resilience
海洋极端气候事件:对生态系统影响和复原力的机械理解
- 批准号:
MR/X023214/1 - 财政年份:2024
- 资助金额:
$ 49.22万 - 项目类别:
Fellowship
Collaborative Research: Mechanistic understanding of chemomechanics in phase-changing electroceramics for sodium-ion batteries
合作研究:钠离子电池相变电陶瓷化学力学的机理理解
- 批准号:
2325463 - 财政年份:2024
- 资助金额:
$ 49.22万 - 项目类别:
Continuing Grant
Imaging Life with Light and Sound: State-of-the-art Photoacoustic Imaging For Mechanistic Understanding Of Human Disease
用光和声音成像生命:最先进的光声成像用于理解人类疾病的机制
- 批准号:
MR/X012549/1 - 财政年份:2023
- 资助金额:
$ 49.22万 - 项目类别:
Research Grant
CAS: Design and Mechanistic Understanding of Emerging Metal Chalcogenide Electrocatalysts for Selective Two-Electron Oxygen Reduction
CAS:用于选择性双电子氧还原的新兴金属硫属化物电催化剂的设计和机理理解
- 批准号:
2247519 - 财政年份:2023
- 资助金额:
$ 49.22万 - 项目类别:
Continuing Grant
ERI: Mechanistic understanding of neural stem cell paracrine activity and extracellular vesicle secretion directed by wireless electrical stimulation
ERI:无线电刺激引导的神经干细胞旁分泌活性和细胞外囊泡分泌的机制理解
- 批准号:
2301908 - 财政年份:2023
- 资助金额:
$ 49.22万 - 项目类别:
Standard Grant
A mechanistic understanding of glymphatic transport and its implications in neurodegenerative disease
对类淋巴运输的机制及其在神经退行性疾病中的影响的理解
- 批准号:
10742654 - 财政年份:2023
- 资助金额:
$ 49.22万 - 项目类别:
Mechanistic Understanding of Multi-scale Sintering Behavior Influenced by Anisotropic Particle and Pore Distributions in Extrusion-based Metal Additive Manufacturing
基于挤压的金属增材制造中受各向异性颗粒和孔隙分布影响的多尺度烧结行为的机理理解
- 批准号:
2224309 - 财政年份:2023
- 资助金额:
$ 49.22万 - 项目类别:
Standard Grant
CAREER: Mechanistic understanding of the nanoscale interactions of structurally tunable 3D assemblies of MXenes-polyelectrolytes
职业:对 MXenes-聚电解质结构可调 3D 组件的纳米级相互作用的机理理解
- 批准号:
2238908 - 财政年份:2023
- 资助金额:
$ 49.22万 - 项目类别:
Standard Grant
Enabling Sulfur-Based Beyond-Lithium Metal Batteries via a Mechanistic Understanding of Advanced Hybrid Cathodes and Borate Electrolytes
通过对先进混合阴极和硼酸盐电解质的机理理解,实现硫基超锂金属电池
- 批准号:
2323065 - 财政年份:2023
- 资助金额:
$ 49.22万 - 项目类别:
Standard Grant