CORE--CELL CULTURE LABORATORY

需要一个集中实验室来提供和维护细胞 对于该计划中的四个项目中的每一个项目。 该核心设施还 将协助制定新的方法和程序 涉及细胞培养。 有十个具体目标：1）培养细胞 研究项目计划的实验所需，2) 传代活性培养物并维持冷冻库存，3) 准备， 对培养基进行灭菌和测试，并保持质量控制，4) 采购、启动和维护项目要求的新细胞系 研究人员，5) 准备人脐静脉的原代培养物 内皮细胞并维持两代，6) 生长 微孔过滤器和微载体珠上的内皮细胞，7) 在微孔过滤器上制备 CaCo-2 培养物，8) 训练新的 组织培养技术方面的科学家计划，并协助项目 研究人员开发新的细胞培养方法 研究问题，9）回顾当前文献并测试新血清， 培养基、生长因子和细胞外基质，以优化 项目研究人员使用的细胞系的生长，以及 10) 开发新系统，开辟新的研究领域 对于该程序。

A centralized laboratory is required to provide and maintain cells for each of the four projects in the Program. This core facility also will assist in the development of new approaches and procedures involving cell culture. There are ten specific aims: 1) Grow cells required for the experiments planned by the research projects, 2) passage active cultures and maintain frozen stocks, 3) Prepare, sterilize and test culture media, and maintain quality control, 4) Procure, initiate and maintain new cell lines requested by program investigators, 5) Prepare primary cultures of human umbilical vein endothelial cells and maintain them for two passages, 6) Grow endothelial cells on micropore filters and microcarrier beads, 7) Prepare CaCo-2 cultures on micropore filters, 8) Train new program scientists in tissue culture techniques, and assist project investigators in the development of new cell culture approaches to research problems, 9) Review current literature and test new sera, media, growth factors, and extracellular matrixes to optimize the growth of cell lines used by the project investigators, and 10) Development new systems that will open up new areas of research for the program.