CORE--LIPID LABORATORY

核心--脂质实验室

• 批准号: 6203299
• 负责人: ARTHUR A. SPECTOR
• 金额: $ 11.34万
• 依托单位: UNIVERSITY OF IOWA
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-08-31 至 2001-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6203299
• 关键词: biomedical facility; chromatography; eicosanoids; fatty acids; mass spectrometry

The purpose of the Core Lipid Laboratory (CLL) is to provide state of the art lipid analysis for the research projects. Discussion between a project investigator and the CLL Director will be held to decide which type of assay would be most suitable and the form in which the samples should be provided to the CLL. The CLL staff will carry out the analytical procedures and provide a written report containing the data as well as an interpretation of the results. Another function of the CLL is developmental. When new areas of investigation are instituted, the CLL will set up any new lipid procedures that will be required. This may involve selecting a procedure available from the literature or, if necessary, developing a new approach. In addition, the CLL will assist the research projects in designing experiments that require extensive lipid analysis. The procedures that will be offered on a routine basis include: 1) fatty acid compositional analysis by gas-lipid chromatography (GLC); 2) analysis of radioactive fatty acids by reverse phase high-performance liquid chromatography (HPLC); 3) chemical preparation of samples for fatty acid GLC and HPLC, including extraction of lipids from tissue or plasma samples, methylation, saponification, silylation, acetylation, and hydrogenation; 4) separation of phospholipids and neutral lipids by thin-layer chromatography (TLC); 5) phospholipid class separation by HPLC; 6) phospholipids molecular species determination by HLPC; 7) eicosanoid separations by HPLC; 8) chemical measurements of the phospholipid, cholesterol, triglyceride, and free fatty acid content; 9) lipid peroxidation, using the thiobarbituric acid reacting substances (TBARS) method for screening purposes, and where positive results are indicated, either ethane collection or lipid hydroperoxide (LOOH) determination by iodometric assay; 10) tocopherol assay by HPLC; and 11) identification of fatty acids and oxidation products by GLC combined with mass spectrometry (MS), negative ion chemical ionization (NICI-MS) to determine the molecular ion, and electron impact (EI-MS) to obtain further structural information.

核心脂质实验室（CLL）的目的是提供 艺术脂质分析的研究项目。 A之间的讨论 项目调查员和CLL主任将举行决定， 类型的测定将是最合适的，样品的形式 应提供给CLL。 CLL工作人员将执行 分析方法，并提供包含数据的书面报告 以及对结果的解释。 CLL的另一个功能 是发展性的。 当建立新的调查领域时， CLL将建立任何新的血脂程序，将需要。 这可能 涉及从文献中选择可用的程序，或者，如果 有必要，开发一种新的方法。 此外，CLL将协助 设计实验的研究项目，需要广泛的 脂质分析 将在常规基础上提供的程序包括： 通过气体-脂质色谱法（GLC）进行脂肪酸组成分析; 2） 反相高效液相色谱法分析放射性脂肪酸 液相色谱法（HPLC）; 3）样品的化学制备 脂肪酸GLC和HPLC，包括从组织或 血浆样品，甲基化，皂化，甲硅烷基化，乙酰化，和 氢化; 4）分离磷脂和中性脂质， 薄层色谱法（TLC）; 5）磷脂类分离， HPLC; 6）通过HLPC测定磷脂分子种类; 7） 通过HPLC分离类二十烷酸; 8）化学测量 磷脂、胆固醇、甘油三酯和游离脂肪酸含量; 9)脂质过氧化，使用硫代巴比妥酸反应物质 （TBARS）方法用于筛查目的，如果阳性结果 指示乙烷收集或脂质过氧化氢（LOOH） 通过碘量法测定; 10）通过HPLC测定生育酚;和 11)GLC结合法鉴定脂肪酸和氧化产物 质谱（MS）、负离子化学电离（NICI-MS） 确定分子离子，并通过电子轰击（EI-MS）获得 更多结构信息