CORE--CELL CULTURE LABORATORY

核心--细胞培养实验室

• 批准号: 6302248
• 负责人: ARTHUR A. SPECTOR
• 金额: $ 22.79万
• 依托单位: UNIVERSITY OF IOWA
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-09-30 至 2000-09-29
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6302248
• 关键词: biomedical facility; cell bank /registry; cell line; human tissue; tissue /cell culture

A centralized laboratory is required to provide and maintain cells for each of the four projects in the Program. This core facility also will assist in the development of new approaches and procedures involving cell culture. There are ten specific aims: 1) Grow cells required for the experiments planned by the research projects, 2) passage active cultures and maintain frozen stocks, 3) Prepare, sterilize and test culture media, and maintain quality control, 4) Procure, initiate and maintain new cell lines requested by program investigators, 5) Prepare primary cultures of human umbilical vein endothelial cells and maintain them for two passages, 6) Grow endothelial cells on micropore filters and microcarrier beads, 7) Prepare CaCo-2 cultures on micropore filters, 8) Train new program scientists in tissue culture techniques, and assist project investigators in the development of new cell culture approaches to research problems, 9) Review current literature and test new sera, media, growth factors, and extracellular matrixes to optimize the growth of cell lines used by the project investigators, and 10) Development new systems that will open up new areas of research for the program.

需要一个集中的实验室来提供和维护细胞 对于该计划中的四个项目中的每个项目。这一核心设施还 将协助开发新的方法和程序 包括细胞培养。有十个具体目标：1)培养细胞 研究项目计划的实验所需，2) 活菌的传代和冷冻保存，3)准备， 对培养物进行灭菌和检测，并保持质量控制，4) 采购、启动和维护计划要求的新细胞系 研究人员，5)准备人脐静脉原代培养 内皮细胞并维持两代，6)生长 微孔滤器和微载体珠上的内皮细胞，7) 在微孔过滤器上准备Caco-2培养物，新培训 为组织培养技术的科学家提供培训，并协助项目 开发新的细胞培养方法的研究人员 研究问题，9)回顾当前文献并测试新的血清， 介质、生长因子和细胞外基质，以优化 项目调查人员使用的细胞系的生长，以及10) 开发新系统，开辟新的研究领域 为了这个项目。