权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

Occurrence and horizontal gene transfer of carbapenemase and ESBL genes in soil microbiomes

碳青霉烯酶和 ESBL 基因在土壤微生物组中的出现和水平基因转移

基本信息

批准号：
NE/N02026X/1
负责人：
Charles William Keevil
金额：
$ 20.18万
依托单位：
University of Southampton
依托单位国家：
英国
项目类别：
Research Grant
财政年份：
2016
资助国家：
英国
起止时间：
2016 至无数据
项目状态：
已结题

来源：
https://gtr.ukri.org/projects?ref=NE%2FN02026X%2F1
关键词：
Occurrence horizontal gene transfer carbapenemase

项目摘要

This project aims to understand the potential of environmental reservoirs and transmission of AMR in members of autochthonous soil microbiomes and between transient allochthonous human and animal pathogens entering the environment, for example from faecal agricultural wastes or domesticated or wild animal and bird faecal ingress. It also seeks to understand if horizontal gene transfer occurs successfully between potential pathogens in the various soil microbiomes and complex matrices encompassing clay, loam and sandy soils. The work will utilize modern molecular biology and genomics to screen microbiome populations and defined third generation ESBL and fourth generation carbapenemase E. coli and Klebsiella pneumoniae donor strains, with an E. coli sensitive strain as the recipient.The University of Southampton team comprises an established scientific group with expertise in survival of faecal pathogens in soil and water, and HGT of ESBL and carbapenemase resistance genes between different species, in collaboration with an Early Career Researcher with expertise in soil microbiology and processes, and stable isotope probing (SIP) to understand community viability, metabolic turnover and gene acquisition. Previous work for UKWIR and Defra/FSA has developed grassed soil microcosms of clay, loam and sandy soils and demonstrated survival of zoonotic pathogens therein for several weeks following faecal waste irrigation. These pathogens were subsequently released from the complex soil matrices using a novel, gentle pulsification procedure followed by membrane filtration and quantitative resuscitation on appropriate selective agar media. This proposal will help identify the specific environmental drivers of the HGT and beta lactamase selection processes, including implications for both anthropogenic (animal husbandry, human wastewater disposal) and non-anthropogenic (wild animal and bird faecal ingress) drivers. The work will also include identifying the implications for pathogens of clinical and/or veterinary importance such Klebsiella pneumonia and E. coli. The research will be able to help inform AMR policy and management strategies, and will form the basis of a subsequent large research proposal to address these policies and strategies more fully.The approaches and methodologies to be developed in this proposal could be translated to other environments, antibacterials or other bacterial communities of interest to the AMR funding bodies.

该项目旨在了解环境水库的潜力和AMR在本地土壤微生物组成员中的传播以及进入环境的短暂外来人类和动物病原体之间的传播，例如来自粪便农业废物或驯养或野生动物和鸟类粪便的进入。它还试图了解在各种土壤微生物组和包括粘土、壤土和桑迪土壤的复杂基质中的潜在病原体之间是否成功地发生水平基因转移。这项工作将利用现代分子生物学和基因组学来筛选微生物群落，并确定第三代ESBL和第四代碳青霉烯酶E。大肠杆菌和肺炎克雷伯菌供体菌株，与E.南安普顿大学的团队包括一个建立的科学小组，该小组具有土壤和水中粪便病原体生存的专业知识，以及不同物种之间ESBL和碳青霉烯酶抗性基因的HGT，与具有土壤微生物学和过程专业知识的早期职业研究人员合作，以及稳定同位素探测（SIP），以了解社区生存能力，代谢周转和基因获得。UKWIR和Defra/FSA先前的工作已经开发了粘土、壤土和桑迪的草土缩影，并证明了粪便灌溉后人畜共患病病原体在其中存活数周。这些病原体随后从复杂的土壤基质中释放，使用一种新的，温和的脉冲程序，然后通过膜过滤和定量复苏适当的选择性琼脂培养基。该提案将有助于确定HGT和β内酰胺酶选择过程的具体环境驱动因素，包括对人为（畜牧业、人类废水处理）和非人为（野生动物和鸟类粪便进入）驱动因素的影响。这项工作还将包括确定临床和/或兽医重要性的病原体，如肺炎克雷伯氏菌和E。杆菌该研究将有助于为AMR政策和管理策略提供信息，并将成为后续大型研究提案的基础，以更全面地解决这些政策和策略。该提案中开发的方法和方法可以转化为AMR资助机构感兴趣的其他环境，抗菌药物或其他细菌群落。