RNA STUDIES AND THE CREUTZFELDT-JACOB DISEASE AGENT

本项目的最终目的是确定克雅氏病的具体代理 （非宿主）分子，即蛋白质和核酸。 是 显然，这些要素的定义对于 最终了解这些代理人和痴呆症， 诱导。 识别真正的试剂特异性分子将 还允许对人类痴呆症进行可靠的诊断测试。 该项目分为两个部分。 第一个涉及一个 确定性评估感染性 试剂，并将用于进一步系统纯化 试剂和试剂蛋白的检测。 第二部分 专注于检测，扩增和测序的特定 可能属于感染原的RNA（cDNA克隆）。

The ultimate aim of this project is to define CJD agent specific (non-host) molecules, i.e. proteins and nucleic acids. It is obvious that definition of these elements will be invaluable for the ultimate understanding of these agents and the dementias they induce. The identification of truly agent specific molecules will also permit reliable diagnostic tests for human dementias. The project is split into two parts. The first deals with a definitive evaluation of the size and density of the infectious agent and will be useful for further systematic purification of agent, and detection of agent protein(s). The second part concentrates on detection, amplification and sequencing of specific RNAs (cDNA clones) that may belong to the infectious agent.