RNA STUDIES AND THE CREUTZFELDT-JACOB DISEASE AGENT

RNA 研究和克雅氏病病原体

• 批准号: 6273895
• 负责人: laura MANUELIDIS
• 金额: $ 16.65万
• 依托单位: YALE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-03-01 至 1999-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6273895
• 关键词: aging; brain; centrifugation; complementary DNA; disease /disorder model; electron microscopy; gel electrophoresis; gene expression; genetic manipulation; hamsters; high performance liquid chromatography; model design /development; molecular cloning; molecular pathology; nervous system infection; nucleic acid hybridization; nucleic acid sequence; pathologic process; phosphorus; prions; radionuclides; radiotracer; spongiform encephalopathy; virus RNA; virus classification; virus protein

The ultimate aim of this project is to define CJD agent specific (non-host) molecules, i.e. proteins and nucleic acids. It is obvious that definition of these elements will be invaluable for the ultimate understanding of these agents and the dementias they induce. The identification of truly agent specific molecules will also permit reliable diagnostic tests for human dementias. The project is split into two parts. The first deals with a definitive evaluation of the size and density of the infectious agent and will be useful for further systematic purification of agent, and detection of agent protein(s). The second part concentrates on detection, amplification and sequencing of specific RNAs (cDNA clones) that may belong to the infectious agent.

这个项目的最终目的是定义特定的克雅氏病代理