CORE--CELL SCIENCES AND IMMUNOCHEMISTRY

核心--细胞科学和免疫化学

• 批准号: 6268481
• 负责人: FELICIA GASKIN
• 金额: $ 18.95万
• 依托单位: UNIVERSITY OF VIRGINIA
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-07-01 至 1999-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6268481
• 关键词: biomedical facility; complementary DNA; cytokine; electron microscopy; enzyme linked immunosorbent assay; epitope mapping; flow cytometry; histology; hybridomas; immunochemistry; immunocytochemistry; immunoprecipitation; in situ hybridization; lymphocyte; messenger RNA; monoclonal antibody; nucleic acid probes; peptide library; protein localization; recombinant proteins; systemic lupus erythematosus

The Cell Sciences and Immunochemistry Core (CIC) is an integral part of the SLE SCOR Center, providing services to the four principal investigators on projects that rely on the following laboratory support. First, routine histology and electron microscopy studies and their interpretations. Second, tissue and cellular localization of antigenic molecules by immunohistology, and messenger RNA (mRNA) by in situ hybridization. Third, acquisition and management of human and murine recombinant proteins and peptide libraries for the "lupus antigens" including Ro60, Ro52, La, Sm, calreticulin, U1-70 RNAP, dsDNA and phospholipids. Fourth acquisition and management of a library of essential monoclonal Ab and cDNA probes for Ag localization and flow cytometry, as well as B cell hybridomas generating sufficient monoclonal Ab for in vivo experiments. Fifth, quantitation and isotype determination of human and murine autoAb by immunoprecipitation and ELISA. Sixth, quantitation of murine cytokines by ELISA. The CIC will function, in collaboration with the project investigators, in experiments that: 1) analyze autoAb responses to the "lupus Ag" and their overlapping peptides, 2) require peptides for mapping their T and B cell epitopes, 3) evaluate the immunopathologic end points including the detection and semi-quantitation glomerulonephritis, sialoadenitis and diseases affecting other organs that result from autoimmune response to the lupus Ag, 4) evaluate the quality of the autoimmune response with respect to the specificity and Ig isotype of the autoAb and the nature of the cytokines produces by peptide specific T cells. In summary, the CIC will support the research project of the SLE SCOR by providing common reagents and by conducting several essential and common techniques.

细胞科学和免疫化学核心（CIC）是一个组成部分， SLE SCOR中心，为四个主要的 研究人员对依赖以下实验室支持的项目进行研究。 首先，常规组织学和电子显微镜研究及其 解释。第二，抗原的组织和细胞定位 分子和信使RNA（mRNA）通过原位 杂交方法第三，人和鼠的采集和管理 “狼疮抗原”的重组蛋白和肽文库 包括Ro 60、Ro 52、La、Sm、钙网蛋白、U1-70 RNAP、dsDNA和 磷脂第四，图书馆的基本采购和管理 用于Ag定位和流式细胞术的单克隆Ab和cDNA探针， 以及B细胞杂交瘤产生足够的单克隆抗体， 实验第五，定量和同种型确定人和 通过免疫沉淀和ELISA检测鼠自身抗体。第六，量化 通过ELISA测定小鼠细胞因子。公民信息中心将与 该项目的研究人员，在实验中：1）分析autoAb 对“狼疮抗原”及其重叠肽的反应，2）需要 用于绘制其T和B细胞表位的肽，3）评估 免疫病理学终点，包括检测和半定量 肾小球肾炎、涎腺炎和影响其他器官的疾病， 结果从自身免疫反应的狼疮抗原，4）评价质量 自身免疫反应的特异性和IG同种型 自身抗体和细胞因子的性质产生的肽特异性 T细胞。总括而言，建造业议会将会支持系统工程学系的研究计划， SCOR通过提供通用试剂和进行几个基本和 常见技术。