CORE--CELL SCIENCES AND IMMUNOCHEMISTRY

核心--细胞科学和免疫化学

• 批准号: 6469205
• 负责人: FELICIA GASKIN
• 金额: $ 21.7万
• 依托单位: UNIVERSITY OF VIRGINIA
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-07-01 至 2002-09-26
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6469205
• 关键词: biomedical facility; complementary DNA; cytokine; electron microscopy; enzyme linked immunosorbent assay; epitope mapping; flow cytometry; histology; hybridomas; immunochemistry; immunocytochemistry; immunoprecipitation; in situ hybridization; lymphocyte; messenger RNA; monoclonal antibody; nucleic acid probes; peptide library; protein localization; recombinant proteins; systemic lupus erythematosus

The Cell Sciences and Immunochemistry Core (CIC) is an integral part of the SLE SCOR Center, providing services to the four principal investigators on projects that rely on the following laboratory support. First, routine histology and electron microscopy studies and their interpretations. Second, tissue and cellular localization of antigenic molecules by immunohistology, and messenger RNA (mRNA) by in situ hybridization. Third, acquisition and management of human and murine recombinant proteins and peptide libraries for the "lupus antigens" including Ro60, Ro52, La, Sm, calreticulin, U1-70 RNAP, dsDNA and phospholipids. Fourth acquisition and management of a library of essential monoclonal Ab and cDNA probes for Ag localization and flow cytometry, as well as B cell hybridomas generating sufficient monoclonal Ab for in vivo experiments. Fifth, quantitation and isotype determination of human and murine autoAb by immunoprecipitation and ELISA. Sixth, quantitation of murine cytokines by ELISA. The CIC will function, in collaboration with the project investigators, in experiments that: 1) analyze autoAb responses to the "lupus Ag" and their overlapping peptides, 2) require peptides for mapping their T and B cell epitopes, 3) evaluate the immunopathologic end points including the detection and semi-quantitation glomerulonephritis, sialoadenitis and diseases affecting other organs that result from autoimmune response to the lupus Ag, 4) evaluate the quality of the autoimmune response with respect to the specificity and Ig isotype of the autoAb and the nature of the cytokines produces by peptide specific T cells. In summary, the CIC will support the research project of the SLE SCOR by providing common reagents and by conducting several essential and common techniques.

细胞科学和免疫化学核心(CIC)是 SLE SCOR中心，为四大校长提供服务 依靠以下实验室支持的项目的调查人员。 首先，常规组织学和电子显微镜研究及其 解读。第二，抗原的组织和细胞定位 分子免疫组织化学和信使核糖核酸(信使RNA)原位检测 杂交。第三，人和鼠的获取和管理 “狼疮抗原”重组蛋白和多肽文库 包括Ro60、Ro52、La、Sm、钙网蛋白、U1-70 RNAP、dsDNA和 磷脂。第四，精品图书馆的采购与管理 用于抗原定位和流式细胞术的单抗和cDNA探针，AS 以及产生足够的体内单抗的B细胞杂交瘤 实验。第五，人类和人类基因的定量和同型测定 免疫沉淀法和酶联免疫吸附试验检测鼠自身抗体。第六，量化 小鼠细胞因子的酶联免疫吸附试验。中投公司将与以下机构合作发挥作用 项目研究人员在以下实验中：1)分析自身抗体 对“狼疮抗原”及其重叠多肽的反应，2)要求 用于绘制其T和B细胞表位的多肽，3)评估 免疫病理终点包括检测和半定量 肾小球肾炎、涎腺炎和影响其他器官的疾病 对狼疮抗原的自身免疫反应结果，4)评估质量 自身免疫应答与特异性和Ig亚型的关系 自身抗体和由多肽特异性产生的细胞因子的性质 T细胞。总而言之，中国投资公司将支持SLE的研究项目 通过提供通用试剂和进行几种基本和 常用技术。