MARINE AND FRESHWATER BIOMEDICAL SCIENCES CENTER

海洋和淡水生物医学科学中心

• 批准号: 2774471
• 负责人: Daniel G Baden
• 金额: $ 2万
• 依托单位: UNIVERSITY OF MIAMI ROSENTEIL SCHOOL
• 依托单位国家: 美国
• 财政年份: 1991
• 资助国家: 美国
• 起止时间: 1991-08-01 至 2001-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2774471
• 关键词: aquatic biology; biomedical facility; environmental toxicology; fish poison; fresh water environment; marine toxins; protozoal toxin; saltwater environment

This proposal is to provide continued funding of a National Institute of Environmental health Sciences Marine and Freshwater Biomedical Sciences Center at the university of Miami. Housed on the Rosenstiel School of Marine and Atmospheric Sciences campus, the Center is a collaborative effort of 10 Center Investigators, 6 Research Investigators, and 5 Adjunct Center Investigators. Three committees oversee Center operation: The Executive Committee, the Internal Advisory Committee, and the External Advisory Committee. Two principal Research Themes form the basis for the interdisciplinary Center work: Marine Toxins and Dietary Risk, and Marine Model Systems of Human Diseases. Both research themes are considered essential to the mission of the University of Miami MFBS Center, and both are within the scope of NIEHS-sponsored research. The first research theme includes Toxin biosynthesis, metabolism, Molecular Pharmacology, Molecular Modeling. Electrophysiology, Orphan Receptors and Charlatan Regulators, Separation Techniques and Assay Methods, and Epidemiology and public Health. The second research theme includes six marine models in various stages of development; Damselfish Neurofibromatosis. Taodfish Hepatic metabolism, Shark Immunology, Aplysia Biology, Transgenic Fishes, and Sentinel Species. Four Pilot Projects have been selected which provide new research directions within the defined research themes; "Immune System Characterization of Atlantic Bottlenose Dolphins", "Aplysia as a Model System for Developmental Neurotoxicology", and "Function of the J-Chain and the Secretory Tail in Immunoglobulin Biosynthesis int he Shark", and "Computational Chemistry of Voltage-Sensitive Sodium Channels: Toxin Effects. As support for the existing individual and collaborative programs 4 Facilities and Service Cores are proposed, based on utilization and overall value to individual programs in Years 1-4 of Center operation; Toxin Probes supplying brevetoxins, saxitoxin, okadaic acid, domoic acid and ciguatoxins and application-related toxin derivatives, In Vitro and in In Vivo Assay Systems which provides a service tissue culture and whole animal approach employing electron microscopy, genetic constructs, computer imaging, and primary and transformed marine cell types. Electrophysiology which provides two separate fully-equipped rigs for toxin work and marine model studies, and Fish and Shellfish Cultivation which provides individual specimens of the model systems for study. A well-defined Community Outreach and Education Program will continue, and implementation of a poison-control center hot lien for seafood intoxication will be undertaken with the Miami Poison Control Center. The University of Miami will continue its commitment to the Center in the form of cost-share. University contributions will be used to fund post-doctoral research associates and their travel and meeting costs, to aid in support of two young faculty who shall act as co-core leaders in Electrophysiology and to provide Center Director travel to Center Director meetings and related expenses. The overall goals of the program in years 6-10 will be to continue to communicate the NIEHS message, to pioneer basic science approaches to human environmental health problems related to natural toxicants, and to continue to search out and characterize new marine models of environment-induced human disease.

该提案旨在为国家研究所提供持续资助 环境健康科学 海洋和淡水生物医学科学 迈阿密大学中心。 坐落于罗森斯蒂尔学院 海洋和大气科学校区，该中心是一个协作 10 名中心研究员、6 名研究人员和 5 名兼职人员的努力 中心调查员。 三个委员会监督中心的运作： 执行委员会、内部咨询委员会和外部 咨询委员会。 两个主要研究主题构成了该研究的基础 跨学科中心工作：海洋毒素和饮食风险以及海洋 人类疾病模型系统。 两个研究主题均被考虑 对于迈阿密大学 MFBS 中心的使命至关重要，而且 属于 NIEHS 资助的研究范围。 第一个研究主题 包括毒素生物合成、代谢、分子药理学、分子生物学 造型。 电生理学、孤儿受体和江湖骗子调节器， 分离技术和检测方法，以及流行病学和公众 健康。 第二个研究主题包括六个不同领域的海洋模型 发展阶段； 雀鲷神经纤维瘤病。 鮟鱇鱼肝 新陈代谢、鲨鱼免疫学、海兔生物学、转基因鱼和 哨兵物种。 已选定四个试点项目，提供新的 既定研究主题内的研究方向； “免疫系统 大西洋宽吻海豚的特征”，“海兔作为模型 发育神经毒理学系统”和“J 链和功能” 鲨鱼免疫球蛋白生物合成中的分泌尾部”，以及 “电压敏感钠通道的计算化学：毒素 效果。 作为对现有个人和协作项目的支持 4 根据利用率和总体情况提出设施和服务核心 对中心运营第 1-4 年各个项目的价值； 毒素 提供短尾毒素、石房蛤毒素、冈田酸、软骨藻酸和 雪卡毒素和与应用相关的毒素衍生物，体外和体内 Vivo Assay Systems 提供组织培养和整体动物服务 采用电子显微镜、基因构建体、计算机的方法 成像，以及原代和转化的海洋细胞类型。 电生理学 提供两个独立的设备齐全的钻机用于毒素工作和海洋 模型研究以及鱼类和贝类养殖，提供个体 用于研究的模型系统的样本。 定义明确的社区 将继续开展外展和教育计划，并实施 毒物控制中心将对海鲜中毒事件进行热留置 与迈阿密毒物控制中心。 迈阿密大学将 继续以费用分摊的形式对该中心做出承诺。 大学捐款将用于资助博士后研究 员工及其旅行和会议费用，以帮助支持两个 年轻教师应作为电生理学的共同核心领导者，并 为中心主任提供参加中心主任会议和相关活动的差旅费 开支。 该计划在第 6-10 年的总体目标将是继续 传达 NIEHS 信息，开创人类基础科学方法 与天然毒物有关的环境健康问题，并继续 寻找并表征环境引起的新海洋模型 人类疾病。