CHROMIUM COATING FOR HIGH RESOLUTION CRYO SEM

用于高分辨率冷冻扫描电镜的铬涂层

• 批准号: 6117263
• 负责人: YA CHEN
• 金额: $ 1.13万
• 依托单位: UNIVERSITY OF WISCONSIN-MADISON
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-09-30 至 2000-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6117263
• 关键词: bioimaging /biomedical imaging; biomedical resource; microscopy; technology /technique

ffigh quality imaging of macromolecular structure of biological samples can be obtained when combining high resolution SEM with advanced thin metal film coating techniques. Because the yield of secondary electron (SE) signal from biological samples is low, a thin layer of fine grain metal film is often necessary to enrich the SE-1 signal yield and enhance the contrast of features of interest. Surface topographic contrast depends on the ratio of SE-USE-11I collection, and the SE-II signal yield depends on the amount and interaction volume of the backscattered electron (BSE) signal (Peters, 1982). Therefore the metals with low BSE yield should be chosen for high resolution SEM coating. Chromium, which has the features of low atomic weight (Z--24) and sufficient SE'signal yield, is an appropriate choice and it was first used by Peters (1982) for high resolution SEM imaging. To create a reproducible pure chromium film, a clean vacuum is the most crucial requirement because Chromium will be oxidized very quickly whenever oxygen exists. Oxidized chromium compound is a totally different metal and is low in the SE yield and low in mass. So, in this project, we will attempt to solve the leaking and oxidation problem so that we can get consistent and clean chromium coating.

生物大分子结构的高质量成像 当结合高分辨率SEM和 先进的金属薄膜镀膜技术。 因为产量 来自生物样品的二次电子（SE）信号是低的，薄的 通常需要一层细粒金属膜来富集SE-1 信号产生并增强感兴趣特征的对比度。 表面形貌对比度取决于SE-USE-11 I的比例 收集，和SE-II信号产量取决于量和 反向散射电子（BSE）信号的相互作用体积（Peters， 1982年）。 因此，应选择具有低BSE产率的金属， 高分辨率SEM涂层。 铬，具有低的特点， 原子量（Z-24）和足够的SE'信号产率，是一种 最早是由Peters（1982）用于高 分辨率SEM成像。 为了制造可再现的纯铬膜， 一个干净的真空是最重要的要求，因为铬将 只要有氧气存在就会很快被氧化。 氧化铬 化合物是完全不同的金属，SE产率低， 质量低。 因此，在这个项目中，我们将尝试解决 泄漏和氧化问题，使我们能够得到一致和清洁 镀铬