GENETIC ANALYSIS OF FEMALE GAMETOPHYTE DEVELOPMENT IN MAIZE

玉米雌配子体发育的遗传分析

• 批准号: 6117325
• 负责人: MATTHEW EVANS
• 金额: $ 1.13万
• 依托单位: UNIVERSITY OF WISCONSIN-MADISON
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-09-30 至 2000-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6117325
• 关键词: bioimaging /biomedical imaging; biomedical resource; growth /development; microscopy; plants

We are using a genetic analysis to study female gametophyte envelopment in maize. The male and female gametes of plants are produced by haploid gametophytes that alternate generations with the diploid sporophyte, the dominant phase of the plant life cycle in angiosperms. After meoisis, the megagametophyte, or embryo sac, is produced by a small number of mitotic divisions and consists of four distinct cells-die egg cell, the central cell, the synergid cell, and the antipodal cell-in characteristic positions within the embryo sac. I propose to identify the developmental defects in embryo sac mutants using confocal microscopy to elucidate the role of the wild-type genes in megagametophyte development.

我们用遗传分析来研究雌配子体 在玉米中种植。 植物的雄配子和雌配子是 由单倍体配子体产生， 二倍体孢子体，植物生活史的优势阶段， 被子植物 减数分裂后，雌配子体，或胚囊， 由少量有丝分裂产生，由四个 不同的细胞-卵细胞，中央细胞，助细胞，和 反足细胞在胚囊内的特征位置。 我建议鉴定胚囊突变体的发育缺陷 使用共聚焦显微镜来阐明野生型基因的作用， 在雌配子体发育中。