NATIONAL RESOURCE FOR APLYSIA

该提案介绍了建立一个国家资源， ACORESIA的总体目标是提供始终如一的高质量 养殖的海兔Aaplasia californica（及其养殖的红藻 （Gracilaria sp.）给国家卫生研究院赞助的研究人员。 A.加州是一个 重要非脊椎动物（后鳃类软体动物）模型系统 健康相关的研究，在神经生理学的行为和 学习 我们将生产动物用于研究，并将进行基本的 研究旨在探索新的模式的用途，并提高 resource. 有四个分项目/具体目标。 1.生产-我们 将使我们1994年的预计产量比1994年增加约225%， 这项赠款的期限，并将使标本从所有生命阶段 价格与实地采集的标本具有竞争力。 2. 遗传学-我们将进行人口遗传学研究，以表征 我们孵化场种群中存在的遗传变异量， 与自然人口相比。 利用这些遗传信息 将为NIH资助的研究者提供杂合性数据 孵化场种群的遗传标记， 可取/不可取的特征。 3.发育神经生理学 该项目将研究神经元兴奋性的调节 影响生殖发育。 单电池电压钳 技术将被应用于研究的发展， 调节刺激作用于离子电流的能力 对调节刺激能力发展的研究， 作用于神经分泌囊细胞中的离子电流。 发展这一 调节能力是性成熟的组成部分。 这 子项目将进一步表征未成熟A. 加利福尼亚州，可能导致一个新的模式使用，并将产生 对孵化场成熟和育种策略有用的信息。 4. 动物健康监测-我们将建立一个监测计划， 筛选水质和动物健康参数，以确保快速 检测和完整记录任何可能 发生在孵化场动物的任何发育阶段。 任何 将对观察到的疾病症状和疑似病原体进行调查 并采取适当的控制措施。

This proposal describes the establishment of a National Resource for Aplysia, whose overall goal is to provide consistently high quality cultured sea hares aplysia californica (and their cultured red algae food Gracilaria sp.) To NIH sponsored researchers. A. Californica is an important non- vertebrate (opisthobranch mollusc) model system for health related research, in the neurophysiology of behavior and learning. We will produce animals for research, and will conduct basic research aimed at exploring new model uses and at improving the resource. There are four sub-project/specific aims. 1. Production- We will increase our projected 1994 production by approximately 225% over the tenure of this grant and will make specimens from all life stages available at a price competitive with field-collected specimens. 2. Genetics- We will perform population genetic studies to characterize the amount of genetic variation present in our hatchery population in comparison to natural populations. The use of this genetic information will be to provide NIH-sponsored investigators with heterozygosity data for the hatchery population and with genetic markers for desirable/undesirable traits. 3. Developmental Neurophysiology-This project will investigate the regulation of neuronal excitability affecting reproductive development. The single cell voltage clamp technique will be applied to the study of the development of the capability of modulatory stimuli to act on ionic currents in applied to the study of the development of the capability of modulatory stimuli to act on ionic currents in neurosecretory bag cells. Development of this modulatory capability is an integral part of sexual maturation. This sub-project will further characterize the neurophysiology of immature A. Californica, potentially leading to a new model use, and will yield information useful to hatchery maturation and breeding strategies. 4. Animal Health Monitoring-We will establish a monitoring program based on screening of water quality and animal health parameters to assure rapid detection and complete documentation of any disease processes that might occur in any developmental stages of animal at the hatchery. Any disease syndromes and suspected pathogens observed will be investigated and appropriate control measures applied.