EICOSANOID METABOLISM IN HUMAN MONOCYTES & ALVEOLAR MACROPHAGE BY HIV INFECTION

人类单核细胞中的二十烷酸代谢

• 批准号: 6274624
• 负责人: MICHAEL J. COFFEY
• 金额: $ 2.15万
• 依托单位: UNIVERSITY OF MICHIGAN AT ANN ARBOR
• 依托单位国家: 美国
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-12-01 至 1998-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6274624
• 关键词: HIV infections; alveolar macrophages; clinical research; diagnostic respiratory lavage; eicosanoid metabolism; helper T lymphocyte; human subject; leukocyte count; lipoxygenase; monocyte; prostaglandin endoperoxide synthase

This study proposes to examine the mechanisms by which HIV increases cyclooxygenase (COX) and decreases 5-lipoxygenase (5-LO) metabolism in 1) monocytes (PBM) 2) alveolar macrophages (AM). The specific aims are to: 1) compare COX and 5-LO metabolism and expression of COX, and 5-LO enzymes in autologous AM and PBM from healthy HIV+ subjects as compared to normal subjects. This will be examined in 2 groups of varying severity as evidenced by the CD4+ T lymphocyte cell count; 2) determine the level of eicosanoids in bronchoalveolar lavage fluid from the HIV+.

这项研究旨在研究艾滋病毒增加的机制， 环氧合酶（考克斯）和减少5-脂氧合酶（5-LO）代谢， 1)单核细胞（PBM）2）肺泡巨噬细胞（AM）。 具体目标是 目的：1）比较考克斯和5-LO的代谢和表达， 来自健康HIV+受试者的自体AM和PBM中的酶进行比较 对正常人来说 这将在2组不同的 严重程度由CD 4 + T淋巴细胞计数证明; 2）确定 HIV阳性者支气管肺泡灌洗液中类花生酸的水平。