Nanoscale interactions of candida species with oral bacteria and surfaces

念珠菌物种与口腔细菌和表面的纳米级相互作用

• 批准号: NE/V019856/1
• 负责人: David Williams
• 金额: $ 1.57万
• 依托单位: CARDIFF UNIVERSITY
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2021
• 资助国家: 英国
• 起止时间: 2021 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=NE%2FV019856%2F1
• 关键词: Nanoscale; interactions; candida; species; oral

The human mouth contains many different types of microorganisms that are often found attached to oral surfaces in 'sticky' communities called biofilms. These microorganisms are held in close proximity and will therefore likely influence the behaviour of each other. The effects of this could result in increased microbial growth, the displacement of some microorganisms to other sites, the alteration of gene expression and potentially, the enabling of microorganisms to cause infection.A PhD research project being done by Ms Megan Williams at the School of Dentistry, Cardiff University has been exploring how a fungus called Candida albicans can interact both with acrylic surfaces (used to manufacture dentures) and also with bacterial species often found alongside Candida albicans. To date, the work has indicated that colonisation of acrylic coated with different fluids, including those generated from tobacco smoking, may change the way Candida albicans grows. Candida albicans can grow as round cells called yeast, or as filamentous forms called hyphae. It is the hyphal forms that are often considered more damaging to human tissue surfaces during infection. In addition, the research shows that when certain bacteria are grown on acrylic surfaces with Candida albicans, hyphal development is also triggered. This is important, as it may mean that occurrence of infection by Candida albicans is at least in part determined by the community composition of the bacteria present alongside Candida. To date, the methods used to study these effects have included fluorescent microscopy, where the Candida is stained to fluoresce a different colour to bacteria and the surface of attachment. Whilst this approach allows quantification of attachment and imaging of the different growth forms, it cannot determine strength of cell-cell-surface interactions. Atomic Force Microscopy (AFM) is a method that provides images through measuring forces acting between a moving probe and a surface. It is possible to attach different molecules and even whole bacteria to the AFM probe, and in doing so, we can measure interactions occurring between bacteria, and either Candida yeast or hyphae serving as the substrate. Dr Laurent Bozec and his team at the University of Toronto are experts in use of AFM, which is not available in the School of dentistry, Cardiff. The exchange therefore offers the PhD student the opportunity to learn a new experimental technique, generate important data for the PhD and benefit from unique networking experiences. The results generated from this proposal will greatly enhance the research output and complement existing findings of the PhD. Ultimately, this could help determine how bacteria physically interact with Candida albicans and trigger the development of hyphal filaments to facilitate infection.

人类口腔中含有许多不同类型的微生物，这些微生物通常以称为生物膜的“粘性”群落附着在口腔表面。这些微生物保持在非常接近的位置，因此可能会影响彼此的行为。这种影响可能导致微生物生长增加，一些微生物转移到其他部位，基因表达改变，并可能使微生物引起感染。梅根威廉姆斯女士在牙科学院正在进行的一项博士研究项目，卡迪夫大学一直在探索一种叫做白色念珠菌的真菌是如何与丙烯酸表面相互作用的（用于制造假牙），也与通常与白色念珠菌一起发现的细菌种类有关。到目前为止，这项工作已经表明，涂有不同液体的丙烯酸的殖民化，包括吸烟产生的液体，可能会改变白色念珠菌的生长方式。白色念珠菌可以生长为圆形细胞称为酵母，或作为丝状形式称为菌丝。在感染过程中，菌丝形式通常被认为对人体组织表面更具破坏性。此外，研究表明，当某些细菌在含有白色念珠菌的丙烯酸表面上生长时，也会引发菌丝发育。这一点很重要，因为这可能意味着白色念珠菌感染的发生至少部分取决于与念珠菌一起存在的细菌的群落组成。到目前为止，用于研究这些影响的方法包括荧光显微镜，其中念珠菌被染色以发出与细菌和附着表面不同的颜色。虽然这种方法允许对不同生长形式的附着和成像进行定量，但它不能确定细胞-细胞-表面相互作用的强度。原子力显微镜（AFM）是一种通过测量作用在移动探针和表面之间的力来提供图像的方法。可以将不同的分子甚至整个细菌附着到AFM探针上，这样做，我们可以测量细菌之间发生的相互作用，并将念珠菌酵母或菌丝作为底物。多伦多大学的Laurent Bozec博士和他的团队是使用AFM的专家，卡迪夫牙科学院没有AFM。因此，交流为博士生提供了学习新的实验技术，为博士生生成重要数据并从独特的网络体验中受益的机会。从这个建议产生的结果将大大提高研究成果，并补充博士现有的研究成果。最终，这可能有助于确定细菌如何与白色念珠菌发生物理相互作用，并引发菌丝丝的发展以促进感染。