ROLE OF MOTOR PROTEINS IN FUNGI MITOSIS

(部分由国家科学基金会MCB 9408249至J.Aist提供支持)。这个项目 目前正在进行中。它的目标是确定部队在哪里 是为了在后期B中分离主轴极而生成的 真菌红假单胞菌，并探讨其作用 有丝分裂、核迁移和核中的细胞质动力蛋白 定位。为了实现前两个目标，激光微束 用于在对照细胞的后期和在 细胞质动力蛋白突变体。要达到后两个目标， 光学陷阱被用来测量逮捕所需的相对力 在对照和突变体中，核迁移和核移位。至 数据显示，纺锤体的两极会自行分离 这一过程是由细胞质介导的。 动力蛋白。这项工作的一部分已被摘录到1997年的ASCB 目前正在编写两篇论文，准备出版。 书名/作者Inoue，S.，O.C.Yoder，B.G.Turjo和J.R.AIST。(1997)细胞质 动力蛋白在真菌中对酯类的形成和功能是必不可少的。 红球线虫Nectria haematocca.莫莱克。比奥尔。8号牢房：

(Supported in part by NSF MCB 9408249 to J. Aist). This project is currently in progress. Its goals are to determine where the forces are generated to separate the spindle poles during anaphase B in the fungus Nectria haematococca, and to investigate the role of cytoplasmic dynein in mitosis, nuclear migration and nuclear positioning. To achieve the first two goals the laser microbeam is used to sever spindles during anaphase in control cells and in cytoplasmic-dynein mutants. To achieve the latter two goals the optical trap is used to measure the relative forces needed to arrest nuclear migration, and displace nuclei, in controls and mutants. To date the data reveal that the spindle poles pull themselves apart during anaphase and that this process is mediated by cytoplasmic dynein. Parts of this work have been abstracted to the 1997 ASCB meeting and two papers are currently being prepared for publication. Inoue, S., O.C. Yoder, B.G. Turgeon and J.R. Aist. (1997) Cytoplasmic dynein is essential for formation and function of asters in the fungus Nectria haematococca. Molec. Biol. Cell 8: