BIOCHEMICAL & BEHAVIOR PROPERTIES OF PRIMARY CILIA: KIDNEY EPITHELIA

（部分由NSF DPP 89 -17375支持。鲍泽）几乎每 人体内的一个细胞含有一个初级纤毛， 从中心体的成熟中心粒开始。 在某些组织中，例如， 在肾上皮细胞中，纤毛从 细胞表面 由于它们的体积小（0.25 lm厚） 初级纤毛难以在体内研究， 仍然未知。 我们已经开发出第一种观看的方法 活细胞中的初级纤毛。 它的基础是在 Formvar薄膜，然后将这些薄膜折叠在两个盖玻片之间。 这使得单个活细胞及其相关的初级 纤毛，要检查沿着与长度相当长的时间，由 视频增强DIC LM。 使用这种方法，我们正在研究 与初级纤毛相关的材料性质和运动性。 我们 也使用低亮度荧光LM，结合 DIC，检查细胞内的表面化学和细胞质交换 初级纤毛 同细胞相关LM/HVEM用于检查 LM结果的超微结构相关性。

(Supported in part by NSF DPP89-17375 to S. Bowser) Almost every cell in the human body contains a single primary cilium that extends from the mature centriole of the centrosome. In some tissues, e.g., kidney epithelia, the cilia projects many micrometers from the cellular surface. Because of their diminutive size (0.25lm thick) primary cilia are difficult to study in vivo and their function remains unknown. We have developed the first method for viewing primary cilia in living cells. It is based on growing kidney cells on Formvar films, and then folding these films between two coverslips. This allows individual living cells, and their associated primary cilia, to be examined along with length for considerable periods by video-enhanced DIC LM. Using this approach we are studying the material properties and motility associated with primary cilia. We are also using low-light level fluorescence LM, in conjunction with DIC, to examine surface chemistry and cytoplasmic exc hange within primary cilia. Same-cell correlative LM/HVEM is used to examine ultrastructural correlates of the LM findings.