CRYSTALLOGRAPHIC STRUCT FUNCT STUDY OF MOESIN, CELLULASES & FLAVO ENZYMES

模蛋白、纤维素酶的晶体结构功能研究

• 批准号: 6281276
• 负责人: P A KARPLUS
• 金额: $ 3.47万
• 依托单位: CORNELL UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-09-15 至 1999-08-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6281276
• 关键词: biological products; biomedical resource; proteins; radiography; structural biology

Within the past year complete MAD data as well as a high resolution (2
) native dataset were collected for Se-Met Moesin crystals and the phase problem has just been solved. Additional monochromatic data sets collected for mutants of pea Ferredoxin Reductase have allowed us to elucidate the binding mode of NADPH at high resolution (~2
). Native data sets have also been collected for Klebsiella aerogenes urease and its mutants, human glutathione reductase, a human cytokine the flt-3 ligand, and a novel flavoenzyme the Staphyboccyl aureus coenzyme A disulfide reductase. All data were collected at low temperature and work on solving and refining these structures is underway.

在过去的一年里，完整的MAD数据以及高 分辨率（2
）收集Se-Met Moesin的天然数据集 晶体和相位问题刚刚得到解决。 额外 豌豆铁氧还蛋白突变体的单色数据集 还原酶使我们能够阐明NADPH的结合模式， 高分辨率（~2
). 还收集了本地数据集， 产气克雷伯氏菌尿素酶及其突变体，人谷胱甘肽 还原酶、人细胞因子FLT-3配体和一种新的黄素酶 金黄色葡萄球菌辅酶A二硫还原酶。 所有数据均 在低温下收集，并致力于解决和精炼这些问题 结构正在进行中。