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QUANTITATIVE TWO PHOTON EXCITED CALCIUM UNCAGING

QUANTITATIVE TWO PHOTON EXCITED CALCIUM UNCAGING
定量二光子激发钙解笼
批准号:
6121948
负责人:
Edward B Brown III
金额:
$0.67万
依托单位:
依托单位国家:
美国
项目类别:
财政年份:
1998
资助国家:
美国
项目状态:
已结题
起止时间:
1998-09-30 至 1999-08-31

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中文摘要
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英文摘要
A technique for the measurement of the two-photon uncaging action cross section of calcium cages has been developed in this laboratory $^{1,2}$. Using this technique, we report on the prognosis for effective two-photon excitation of a novel calcium cage, DMNPE-4 $^{3}$. We also describe calculations which predict the total amount of calcium ions uncaged and the temporal behavior of the liberated calcium distribution under given multiphoton photolysis conditions with a given cage. These calculations, combined with the two-photon uncaging action cross sections that we have measured for a variety of calcium cages enable the quantitative study of calcium dependent processes with high spatial resolution within cells or thick tissue samples. The ultimate intent of these studies is the application of these tools to the quantitative study of the spontaneous transient calcium concentration elevations ("sparks") in developing skeletal muscle cells, probing the potentiation/inhibition relation ships between consecutive sparks in one localized region as well as between sparks in spatially separate regions. 1. Shear, J. et al. {\em Biophys. J.}70(2) A211 (1996) 2. Brown et al, Manuscript in Preparation (1997) 3. Ellis-Davies, G.{\em Biophys. J.}72(2) A246 (1997)
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OPTICAL MEASUREMENT OF TRANSPORT DEEP INSIDE TUMORS
  • 批准号:
    6436414
  • 项目类别:
  • 资助金额:
    $4.02万
  • 财政年份:
    2001
  • 负责人:
    Edward B Brown III
  • 依托单位:
OPTICAL MEASUREMENT OF TRANSPORT DEEP INSIDE TUMORS
  • 批准号:
    6208459
  • 项目类别:
  • 资助金额:
    $3.24万
  • 财政年份:
    2000
  • 负责人:
    Edward B Brown III
  • 依托单位:
DENDRITIC SPINES: QUANTITATIVE MPE CALCIUM UNCAGING TO DEEP TISSUES
  • 批准号:
    6349456
  • 项目类别:
  • 资助金额:
    $1.3万
  • 财政年份:
    2000
  • 负责人:
    Edward B Brown III
  • 依托单位:
DENDRITIC SPINES: QUANTITATIVE MPE CALCIUM UNCAGING TO DEEP TISSUES
  • 批准号:
    6206190
  • 项目类别:
  • 资助金额:
    $1.3万
  • 财政年份:
    1999
  • 负责人:
    Edward B Brown III
  • 依托单位:
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