QUANTITATIVE TWO PHOTON EXCITED CALCIUM UNCAGING

一种测量双光子释放作用的技术 本实验室研制了钙笼的横截面 $^{1，2}$。 使用这种技术，我们报告的预后， 新型钙笼DMNPE-4有效双光子激发 $^{3}$。 我们还描述了预测总量的计算 释放的钙离子和释放的钙离子的时间行为 在给定的多光子光解条件下的钙分布 一个特定的笼子。 这些计算，结合双光子 我们已经测量了各种各样的释放作用截面， 钙笼使得能够定量研究钙依赖性 在细胞或厚组织内具有高空间分辨率的突起 样品 这些研究的最终目的是应用 这些工具的定量研究的自发瞬变 发育中的骨骼中钙浓度升高（“火花”） 肌细胞，探索增强/抑制关系 在一个局部区域中的连续火花之间以及 在空间上分离的区域中产生火花。 1. Shear，J. et al. Biophys. J.} 70（2）A211（1996）2. Brown等人，Mandarin 03.《准备工作》（1997） Ellis-Davies，G.生物物理学。 J.} 72（2）A246 （一九九七年）

A technique for the measurement of the two-photon uncaging action cross section of calcium cages has been developed in this laboratory $^{1,2}$. Using this technique, we report on the prognosis for effective two-photon excitation of a novel calcium cage, DMNPE-4 $^{3}$. We also describe calculations which predict the total amount of calcium ions uncaged and the temporal behavior of the liberated calcium distribution under given multiphoton photolysis conditions with a given cage. These calculations, combined with the two-photon uncaging action cross sections that we have measured for a variety of calcium cages enable the quantitative study of calcium dependent processes with high spatial resolution within cells or thick tissue samples. The ultimate intent of these studies is the application of these tools to the quantitative study of the spontaneous transient calcium concentration elevations ("sparks") in developing skeletal muscle cells, probing the potentiation/inhibition relation ships between consecutive sparks in one localized region as well as between sparks in spatially separate regions. 1. Shear, J. et al. {\em Biophys. J.}70(2) A211 (1996) 2. Brown et al, Manuscript in Preparation (1997) 3. Ellis-Davies, G.{\em Biophys. J.}72(2) A246 (1997)