WHOLE MEMBRANE CAPACITANCE MEASUREMENTS OF RBL CELLS DURING SECRETION

RBL 细胞分泌期间的全膜电容测量

• 批准号: 6121874
• 负责人: RUSSELL THOMPSON
• 金额: $ 3.44万
• 依托单位: CORNELL UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-09-30 至 1999-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6121874
• 关键词: animal tissue; bioengineering /biomedical engineering; biomedical resource; human tissue; immunology; inflammation; lipids; lymphatic system

While RBL cells are common system for studying secretion, existing data seems to indicate that the plasma membrane area does not change, contrary to the results in a number of other secretory cells. However, the data are based on whole cell capacitance measurements (under conditions in which RBL cells have been found not to secrete), and on serial electron microscopy reconstruction of cells (which is a less accurate technique than capacitance measurements). We propose to do permeabilized patch capacitance recording of RBL cells, thus allowing electrical access to the whole cell membrane while maintaining the cytoplasmic integrity necessary to have secretion.

而RBL细胞是研究分泌物的常见系统，现有 数据似乎表明质膜面积没有变化， 与其他一些分泌细胞的结果相反。 然而，这些数据是基于整个电池的电容测量 (在发现RBL细胞不分泌的情况下)， 以及细胞的连续电子显微镜重建(这是一种 技术不如电容测量准确)。我们建议 做RBL细胞的通透性膜片电容记录，从而 允许电接触整个细胞膜，同时 维持分泌所必需的细胞质完整性。