TECHNIQUE FOR SINGLE PARTICLE LOCALIZATION & TRACKING IN FLUORESCENCE MICROSCOPE

单粒子定位技术

• 批准号: 6349389
• 负责人: RUSSELL THOMPSON
• 金额: $ 1.06万
• 依托单位: CORNELL UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-09-01 至 2001-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6349389
• 关键词: bioengineering /biomedical engineering; biological products; biomedical equipment development; biomedical resource; lasers; technology /technique

We established a FCS setup that easily allows the combination of one- and two-photon excitation schemes without changing major optical components. By proper choice of the confocal detection system, such as pinhole diameters, volume elements of equal size can be realized what allows for an easy comparison of dye characteristics such as excitation cross-sections with both excitation alternatives on a single molecule level.

我们建立了一个 FCS 设置，可以轻松地组合 一光子和双光子激发方案，无需改变主要光学 成分。 通过正确选择共焦检测系统，这样 随着针孔直径的增加，可以实现相同尺寸的体积单元 可以轻松比较染料特性，例如 励磁截面与两种励磁替代方案 单分子水平。