MEMBRANE DYNAMICS OF FUSION PROCESSES

融合过程的膜动力学

• 批准号: 6121877
• 负责人: RUSSELL THOMPSON
• 金额: $ 4.7万
• 依托单位: CORNELL UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-09-30 至 1999-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6121877
• 关键词: bioengineering /biomedical engineering; biomedical resource; human tissue; lipids; technology /technique

Work has continued on the effort to measure the fusion of single vesicles to planar membranes. Systems have been developed for the formation of large unilamellar vesicles and planar membranes, and we are working towards reliably patch clamping them. A novel electrical configuration has been adopted in which the pipette and surrounding bath are maintained at ground potential, and the solution on the trans side of the bilayer is stimulated with an a.c. voltage. This configuration sidesteps the normal problems associated with large pipette and membrane capacitances and provides for a nearly background free measurement limited only by amplifier noise.

工作继续努力，以衡量融合的单一 囊泡到平面膜。 系统已经开发， 形成大的单层囊泡和平面膜，我们 正在努力实现可靠的膜片钳技术。 一种新颖的电气 已经采用了这样的配置，其中移液管和周围环境 浴保持在地电位，并在反式上的溶液 用a.c.电压. 这 配置回避了与大型 移液管和膜电容，并提供了一个几乎背景 自由测量仅受放大器噪声限制。