PATCH CAPACITANCE RECORDING OF RBL CELLS DURING SECRETION

RBL 细胞分泌期间的贴片电容记录

• 批准号: 6121875
• 负责人: RUSSELL THOMPSON
• 金额: $ 8.32万
• 依托单位: CORNELL UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-09-30 至 1999-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6121875
• 关键词: animal tissue; bioengineering /biomedical engineering; biomedical resource; human tissue; immunology; inflammation; lipids; lymphatic system

A patch clamp capacitance recording system has been set up with a resolution of 0.1 fF, allowing high resolution measurements of secretion in the patch. We have applied this system to RBL cells, a cell line commonly used for studying secretion. While we have seen endocytosis (downward steps in capacitance), no exocytotic steps have been observed during secretion. Instead, we see spikes in the capacitance, possibly indicating transient fusion events where the vesicle fuses only briefly with the plasma membrane. Work is underway to confirm the nature of the capacitance spikes and explore their dependence on external calcium and other conditions necessary for secretion

建立了膜片钳电容记录系统， 分辨率为0.1 fF，允许高分辨率测量 贴片中的分泌物。 我们已经将该系统应用于RBL细胞， 通常用于研究分泌的细胞系。 虽然我们已经看到 内吞（电容的向下步骤），没有胞吐步骤， 在分泌过程中观察到。 相反，我们看到 电容，可能表明瞬时融合事件，其中 囊泡仅与质膜短暂融合。 工作正在进行 以确认电容尖峰的性质并探索其 依赖于外部钙和其他必要的条件， 分泌