INTRINSIC FLUORESCENCE OF TRYPTOPHAN MUTANTS OF ADENYLATE KINASE

我们已经制备了四种腺苷酸激酶的色氨酸突变体 Ecofi(S41瓦、F86瓦、Y133瓦、F137瓦)。它的荧光性质 这些突变体非常多样化，表明存在广泛的本地化 色氨酸报告基团的构象可变性。为 例如，F86W和S41W分别是排放的最大范围 325~357 nm，寿命3~7 ns。

We have prepared four tryptophan mutants of adenylate kinase from Ecofi (S41 W, F86W, Y133W,F137W). The fluorescence properties of these mutants are very diverse indicating a wide range of localized conformational variability for the tryptophan reporter groups. For example, the F86W and S41W, respectively, the emission maximum range from 325 to 357 nm and the lifetime from 3 to 7 ns.