UV RESONANCE RAMAN CROSS SECTION MEASUREMENT OF BACTERIAL CELLS

该项目的目标是开发紫外共振拉曼 细菌快速检测与鉴定的光谱分析技术 病原体。作为第一步，我们正在确定 不同细菌样品光谱的共振拉曼光谱 不同细菌细胞散射截面的测量 台阶。到目前为止，我们已经测量了各种细菌的拉曼光谱 包括大肠埃希菌、表皮葡萄球菌、枯草芽孢杆菌和芬迪葡萄球菌 用251 nm测量了这些样品的拉曼散射截面 激动人心。为了计算横截面，我们使用了钠 硫酸盐作为内标。已测量的横截面为 细菌细胞在不同的条件下处于三个阶段，即滞后、对数和静止 稀释条件。类似的实验将在其他地方进行 激发波长包括241 nm和230 nm。

The goal of this project is to develop UV resonance Raman spectroscopy for rapid detection and identification bacterial pathogens. As a first step we are in the process of characterizing resonance Raman of the spectra of various bacterial samples and measuring scattering cross section of bacterial cells of various steps. So far we have measured Raman spectra of various bacteria including E. coli, S. Epidermidis, B. subtilis and C. feundie and measured Raman cross section for these samples using 251 nm excitation. In order to calculate cross section we are using sodium sulfate as an internal standard. Cross sections have been measured of bacterial cells in three phases, lag, log and stationary under various dilution conditions. Similar experiments will be carried at other excitation wavelengths including 241nm and 230 nm.