UV RESONANCE RAMAN CROSS SECTION MEASUREMENT OF BACTERIAL CELLS

细菌细胞的紫外共振拉曼截面测量

• 批准号: 6314181
• 负责人: WILFRED NELSON
• 金额: $ 1.38万
• 依托单位: MASSACHUSETTS INSTITUTE OF TECHNOLOGY
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-06-01 至 2001-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6314181
• 关键词: bacteria; bioengineering /biomedical engineering; biomedical resource; technology /technique

The goal of this project is to develop UV resonance Raman spectroscopy for rapid detection and identification bacterial pathogens. As a first step we are in the process of characterizing resonance Raman of the spectra of various bacterial samples and measuring scattering cross section of bacterial cells of various steps. So far we have measured Raman spectra of various bacteria including E. coli, S. Epidermidis, B. subtilis and C. feundie and measured Raman cross section for these samples using 251 nm excitation. In order to calculate cross section we are using sodium sulfate as an internal standard. Cross sections have been measured of bacterial cells in three phases, lag, log and stationary under various dilution conditions. Similar experiments will be carried at other excitation wavelengths including 241nm and 230 nm.

本项目的目标是发展紫外共振拉曼光谱 用于快速检测和鉴定细菌光谱学 病原体 作为第一步，我们正在描述 各种细菌样品光谱的共振拉曼， 测量各种细菌细胞的散射截面 步 到目前为止，我们已经测量了各种细菌的拉曼光谱 包括大肠coli、S.表皮，B。枯草芽孢杆菌和C. feundie和 使用251 nm波长测量这些样品的拉曼截面 激发 为了计算横截面，我们使用钠 硫酸盐作为内标。 已经测量了横截面， 细菌细胞在三个阶段，滞后，对数和静止在各种 稀释条件 类似的实验将在其他 激发波长包括241 nm和230 nm。