UV RESONANCE RAMAN CROSS SECTION MEASUREMENT OF BACTERIAL CELLS

该项目的目标是开发紫外共振拉曼光谱 快速检测和鉴定细菌的光谱学 病原体。 作为第一步，我们正在表征 各种细菌样品光谱的共振拉曼 测量各种细菌细胞的散射截面 步骤。 到目前为止我们已经测量了多种细菌的拉曼光谱 包括大肠杆菌、表皮葡萄球菌、枯草芽孢杆菌和 C. feundie 以及 使用 251 nm 测量这些样品的拉曼截面 激发。 为了计算横截面，我们使用钠 硫酸盐作为内标。 横截面已测量 细菌细胞在不同条件下处于三个阶段：滞后期、对数期和静止期 稀释条件。 类似的实验将在其他地方进行 激发波长包括241nm和230nm。

The goal of this project is to develop UV resonance Raman spectroscopy for rapid detection and identification bacterial pathogens. As a first step we are in the process of characterizing resonance Raman of the spectra of various bacterial samples and measuring scattering cross section of bacterial cells of various steps. So far we have measured Raman spectra of various bacteria including E. coli, S. Epidermidis, B. subtilis and C. feundie and measured Raman cross section for these samples using 251 nm excitation. In order to calculate cross section we are using sodium sulfate as an internal standard. Cross sections have been measured of bacterial cells in three phases, lag, log and stationary under various dilution conditions. Similar experiments will be carried at other excitation wavelengths including 241nm and 230 nm.