RAPID DETECTION OF TOXIC ALGAE USING UV RESONANCE RAMAN

使用紫外共振拉曼快速检测有毒藻类

• 批准号: 6314182
• 负责人: WILFRED NELSON
• 金额: $ 1.38万
• 依托单位: MASSACHUSETTS INSTITUTE OF TECHNOLOGY
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-06-01 至 2001-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6314182
• 关键词: biological products; biomedical resource; infection; microorganism

The objective of this project to rapidly detect and identify toxin producing algae. Many types of algae are capable of producing toxins such as domoic acid. Since, domoic acid has well characterized UV visible absorption, UV resonance Raman spectroscopy can be used to selectively excite and observe domoic acid in algae. We have studied three kinds of algae Pseudonitzichia N95K, F996B, and V11B using 251 nm laser excitation. A series of standard domoic acid spectra were also obtained in the concentration range varying from 6 ppm to 96 ppb. Cross section of domoic acid 1640 cm-1 were also obtained by sodium sulfate as an internal standard.

该项目的目标是快速检测和鉴定毒素 产生藻类。许多类型的藻类都能产生毒素 如软骨藻酸。自那以后，软骨藻酸很好地表征了紫外线 可见吸收、紫外共振拉曼光谱可用于 选择性地激发和观察藻类中的软骨藻酸。我们已经研究过 利用251分离N95K、F996B和V11B三种藻类 NM激光激励。得到了一系列软骨藻酸的标准光谱。 也可在6ppm至96ppb的浓度范围内获得。 软骨藻酸的截面积为1640 cm~(-1)。 硫酸盐作为内标。