RAPID DETECTION OF TOXIC ALGAE USING UV RESONANCE RAMAN

使用紫外共振拉曼快速检测有毒藻类

• 批准号: 6281792
• 负责人: WILFRED NELSON
• 金额: $ 0.38万
• 依托单位: MASSACHUSETTS INSTITUTE OF TECHNOLOGY
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-06-01 至 1999-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6281792
• 关键词: biological products; biomedical resource; infection; microorganism

The objective of this project to rapidly detect and identify toxin producing algae. Many types of algae are capable of producing toxins such as domoic acid. Since, domoic acid has well characterized UV visible absorption, UV resonance Raman spectroscopy can be used to selectively excite and observe domoic acid in algae. We have studied three kinds of algae Pseudonitzichia N95K, F996B, and V11B using 251 nm laser excitation. A series of standard domoic acid spectra were also obtained in the concentration range varying from 6 ppm to 96 ppb. Cross section of domoic acid 1640 cm-1 were also obtained by sodium sulfate as an internal standard.

本项目的目的是快速检测和识别毒素， 产生藻类。 许多种类的藻类都能产生毒素 例如软骨藻酸。 软骨藻酸已充分表征紫外 可见光吸收、UV共振拉曼光谱可用于 选择性地激发和观察藻类中的软骨藻酸。 我们研究了 用251 nm激光激发。 一系列标准软骨藻酸光谱， 也可以在6ppm至96 ppb的浓度范围内获得。 软骨藻酸的横切面为1640 cm-1， 硫酸盐作为内标。