IN VIVO IN UTERO MICROSCOPIC MRI OF RAT FETUS W/ CONGENITAL DIAPHRAGMATIC HERNIA

患有先天性膈疝的大鼠胎儿的体内子宫显微 MRI

• 批准号: 6121057
• 负责人: ALISON K HOYDU
• 金额: $ 2.44万
• 依托单位: UNIVERSITY OF PENNSYLVANIA
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-09-30 至 1999-09-29
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6121057
• 关键词: Mammalia; biomedical resource; congenital disorders; connective tissue; growth /development; infant mortality; liver; low birth weight infant human; magnetic resonance imaging; mother /infant health care; respiratory system; surgery; technology /technique

In Utero surgical techniques for the reversal of anatomical malformations are developed in small mammalian models. The design of new techniques suffers from lack of non-invasive pre- and post-surgical fetal monitoring. We have applied high resolution MRI to in-vivo, in-utero imaging of a rat model for Congenital Diaphragmatic Hernia (CDH). CDH is a developmental anomaly which involves incomplete closure of the diaphragm, herniation of the liver and abdominal viscera into the thoracic cavity, and lung hypoplasia. Eight dams were imaged on days 19-22 of gestation (once a day) to diagnose the presence or absence of CDH and monitor the effects of surgery. Those who were shown to be CDH+ on day 19 underwent immediate surgical tracheal ligation to reverse pulmonary hypoplasia and force the abdominal contents from the thoracic cavity. 39 rat fetuses were imaged using a multislice, T2 weighted, fast spin echo sequence on a 4T whole body imaging system (GE, Signa). Pathology and results of surgery were confirmed post-mortem by high resolution imaging (9.4T) and subsequent microscopic dissection. This information will help in the use and development of in utero intervention for treatment of congenital abnormalities.

子宫内解剖反转的外科技术