MEASUREMENT OF MOLECULAR DISTANCES USING DIPOLAR COUPLED NITROXIDE SPIN LABELS

使用偶极耦合氮氧化物自旋标记测量分子距离

• 批准号: 6120685
• 负责人: ERIC J HUSTEDT
• 金额: $ 1.08万
• 依托单位: UNIVERSITY OF ILLINOIS AT CHICAGO
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-04-15 至 1999-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6120685
• 关键词: biomaterials; biomedical resource; computers; technology /technique

CW-EPR spectra of N6-spin-labeled coenzyme NAD+ (SL-NAD+) bound to microcrystalline, tetrameric glyceraldehyde-3-phosphate dehydrogenase (GAPDH) have been collected at 9.8, 34, and 94 GHz. These data have been analyzed, using a combination of the methods of simulated annealing and global analysis, employing a model with pairwise R-axis related dipolar interactions between the nitroxides of N6-SL-NAD+ bound to the enzyme tetramer. Using this approach, a unique fit to the data has been obtained which is consistent with a molecular model built from the known crystal structure of the DAPDH tetramer.

n6自旋标记辅酶NAD+ (SL-NAD+)结合的CW-EPR谱