LABELING OF ADENOVIRUS 12 KNOB

腺病毒蛋白，A12，参与了结合的 病毒到受体。 该蛋白已被克隆与6-组氨酸 用于纯化的尾和用于除去His-尾的凝血酶位点。 一 合成NiNTA-纳米金探针并用该蛋白进行测试， 其是64 kDa的三聚体（良好的STEM样品）。 的 凝血酶裂解的蛋白质用作对照。 几乎所有的蛋白质 用his-尾巴标记，通常用2-3个金簇， 与三聚体一致 通过STEM，非His蛋白质 （切割的）显示没有簇结合。

The adenovirus protein, A12, is involved in the binding of the virus to the receptor. The protein has been cloned with 6-histidine tail for purification and a thrombin site to remove the his-tail. A NiNTA-Nanogold probe was synthesized and tested with this protein, which is a trimer of 64 kDa (a good STEM sample). The thrombin-cleaved protein was used as a control. Nearly every protein with the his-tail was labeled, often with 2-3 gold clusters, consistent with it being a trimer. By STEM, the non-his protein (cleaved) showed no cluster binding.