人类蛋白质差异显示测定 ICAT-ULC-MS/MS-猫眼课题宝

人类蛋白质差异显示测定 ICAT-ULC-MS/MS

批准号：

6311048

负责人：

Babak Esmaeli-Azad

金额：

$14.31万

依托单位：

BIOARRAY TECHNOLOGIES, INC.

依托单位国家：

美国

项目类别：

财政年份：

2001

资助国家：

美国

项目状态：

已结题

起止时间：

2001-08-01 至 2002-01-31

来源：

https://reporter.nih.gov/project-details/6311048

关键词：

differential display technique immunoaffinity chromatography mass spectrometry neoplastic cell phosphorylation proteomics technology /technique development tissue /cell culture tyrosine

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DESCRIPTION: (Applicant's description) Development and testing of a protein differential display assay system, for proteomes of higher eukaryotes, using isotope-coded affinity tags (ICAT) and microcapillary liquid chromatography tandem mass spectrometry (uLC-MS/MS), is proposed. This system includes specific immunoaffinity capture of proteins during CAT analysis. Initial Phase I studies are proposed for development and optimization of phosphotyrosine specific protein differential display assay using a well characterized phosphotyrosine specific antibody and human carcinoma cells in culture. In Phase II, assays for other cell types and tissues will be developed. In addition, subtraction of high abundant and enrichment of low abundant proteins, in crude protein extracts, will be incorporated. Phase II studies also include use of novel CATs for comprehensive analysis of post-translational modifications.ln full development, this assay system provides for comprehensive analysis of the complex proteomes such as that of human. PROPOSED COMMERCIAL APPLICATION: Not available

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