Online 2-D Proteomics Differential Display

描述(由申请人提供)：拟议研究的目标和 开发的目的是创建一个完整的在线数字化实验室原型 一种用于二维凝胶电泳多色分析的检测系统 以蛋白质组学为基础。第一阶段的可行性研究表明，这是有能力的。 检测和定量单个蛋白质斑点的原型系统 通过二维SDS-聚丙烯酰胺凝胶电泳法进行迁移。在……里面 全面开发，这个在线系统可以绘制所有蛋白质的地图 有机体、细胞或组织中可能存在的丰度水平。多色 蛋白质制图可实现准确的蛋白质差异显示领先 以生成关于生物范例的功能信息。这种类型 对于充分了解基因的功能是必要的 以及他们在后基因组时代的产品。 建议的商业应用： 不可用

DESCRIPTION (provided by applicant): The goal of the proposed research and development is to create a completed laboratory prototype online digital detection system for multi-color analysis of 2-dimensional gel electrophoresis based proteomics. Phase I feasibility studies have shown capability of this prototype system to detect and quantitate individual protein spots as they migrate through the 2nd dimension SDS-Polyacrylamide Gel Electrophoresis. In full development, this online system makes cartography of proteins of all abundance levels in an organism, cell or tissue possible. Multi-color cartography of proteins permits accurate protein differential display leading to generation of functional information about biological paradigms. This type of information is necessary for the full understanding of the function of genes and their products in the post-genome era. PROPOSED COMMERCIAL APPLICATION: Not Available