Online 2-D Proteomics Differential Display

在线二维蛋白质组差异显示

• 批准号: 6338292
• 负责人: Babak Esmaeli-Azad
• 金额: $ 50.94万
• 依托单位: BIOARRAY TECHNOLOGIES, INC.
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-05-10 至 2003-03-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6338292
• 关键词: SDS polyacrylamide gel electrophoresis; biomedical equipment development; biotechnology; cadmium; differential display technique; digital imaging; gel electrophoresis; high throughput technology; information display; online computer; protein sequence; protein structure function; proteomics; radiation detector; radionuclide imaging /scanning; radionuclides; tellurium; zinc

DESCRIPTION (provided by applicant): The goal of the proposed research and development is to create a completed laboratory prototype online digital detection system for multi-color analysis of 2-dimensional gel electrophoresis based proteomics. Phase I feasibility studies have shown capability of this prototype system to detect and quantitate individual protein spots as they migrate through the 2nd dimension SDS-Polyacrylamide Gel Electrophoresis. In full development, this online system makes cartography of proteins of all abundance levels in an organism, cell or tissue possible. Multi-color cartography of proteins permits accurate protein differential display leading to generation of functional information about biological paradigms. This type of information is necessary for the full understanding of the function of genes and their products in the post-genome era. PROPOSED COMMERCIAL APPLICATION: Not Available

描述（由申请人提供）：拟议研究的目标和 开发是创建一个完整的实验室原型在线数字化 二维凝胶电泳多色分析检测系统 基于蛋白质组学。第一阶段的可行性研究已经表明了这种能力 用于检测和定量单个蛋白质点的原型系统 通过二维 SDS 聚丙烯酰胺凝胶电泳进行迁移。在 全面发展，这个在线系统可以对所有蛋白质进行制图 生物体、细胞或组织中可能的丰度水平。多色的 蛋白质制图允许准确的蛋白质差异显示 生成有关生物范式的功能信息。这种类型 信息对于充分理解基因的功能是必要的 以及他们在后基因组时代的产品。 拟议的商业应用： 无法使用