Phototoxicity using the Chorioallantoic Membrane

使用绒毛尿囊膜的光毒性

• 批准号: 6337940
• 负责人: DANIEL R CERVEN
• 金额: $ 10万
• 依托单位: MB RESEARCH LABORATORIES, INC.
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-06-01 至 2003-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6337940
• 关键词: alternatives to animals in research; chorioallantoic membrane; cytotoxicity; drug adverse effect; drug screening /evaluation; irritation /irritant; photochemistry; technology /technique development; tissue /cell culture

DESCRIPTION (Applicant's Abstract): This proposal, "Phototoxicity Screen using the Chorioallantoic Membrane" (PhotoCAM) is an alternative phototoxicity screening test to identify chemical mediators and substances with phototoxic properties, which is in demand by the pharmaceutical, chemical, and consumer product industries. The chorioallantoic membrane (CAM) of the fertile chicken egg has already shown to be a useful, sensitive model for detecting irritants. The purpose of this proposal is to demonstrate the feasibility of employing the CAM assay, as well as endpoints of PGE2 and cytokine production by the epidermal cells comprising the tissue, as test systems to identify potential phototoxins or photoirritants. There have only been two papers published in the literature using this model, thus this is a relatively novel application of this model system. The use of an intact tissue has several advantages over existing cell culture-based phototoxicity screening assays. These include the presence of multiple cell types found in the skin, and a readily accessible circulatory system that is in contact with metabolically functional hepatic tissue. The PhotoCAM Assay described herein will provide a sensitive test system while (1) complying with the Animal Welfare Act (AWA) by directly addressing the 3Rs, by reducing animal usage; (2) increasing the discriminating power and quality of data generated when compared to cell culture methods; and (3) greatly reducing the costs associated with the use of animals for phototoxicity screening studies.

描述（申请人的摘要）：该提案“使用光毒性筛选 绒毛尿囊膜”（PhotoCAM）是另一种光毒性 筛选试验以确定具有光毒性的化学介质和物质 制药、化学品和消费者所需要的特性 产品行业。受精鸡的绒毛尿囊膜 (CAM) 鸡蛋已被证明是检测刺激物的有用且灵敏的模型。 本提案的目的是证明采用该方案的可行性 CAM 测定，以及 PGE2 和细胞因子产生的终点 构成组织的表皮细胞，作为识别潜力的测试系统 光毒素或光刺激物。仅在该杂志上发表过两篇论文 文献使用该模型，因此这是一个相对新颖的应用 这个模型系统。与使用完整的组织相比，使用完整的组织有几个优点： 现有的基于细胞培养的光毒性筛选测定。这些包括 皮肤中存在多种细胞类型，并且易于获取 与具有代谢功能的肝脏接触的循环系统 组织。本文描述的 PhotoCAM 检测将提供灵敏的测试 系统，同时 (1) 直接遵守《动物福利法》(AWA) 通过减少动物使用来解决 3R 问题； （二）加大歧视力度 与细胞培养方法相比，生成的数据的功效和质量；和 (3) 大大降低使用动物的成本 光毒性筛选研究。