CA2+ TRANSPORT FUNCTION OF CHORIOALLANTOIC MEMBRANE

本应用程序的总体目标是了解法规 胚胎钙代谢的过程，特别强调 经胎盘钙转运 所选系统的建议 研究的是鸡胚绒毛尿囊膜（CAM）， 代表相当于胎盘单位的鸟类， 将蛋壳钙转移到胚胎循环中 在发展过程中。 本建议书所述的项目旨在 阐明生物化学，细胞和分子机制调节 CAM钙转运功能。 我们的方法是研究 转运相关蛋白（例如钙结合蛋白，CaBP;和 Ca 2 +-ATP酶）作为功能标记物 钙转运过程的分子。 具体来说，我们计划 首先在亚细胞水平上表征和定位这些组分， 分析其在CAM钙离子代谢中的功能参与和机制作用 运输 其次，我们将研究细胞，生物化学， 正常期间导致CaBP表达调节的分子事件 胚胎发育和实验诱导条件下， 维生素K和/或钙缺乏。 我们相信这些实验 这里提出的将定义这些标记分子的功能作用， CAM钙转运和提供有关机制的基本信息 调节重要的生理功能，经胎盘钙 运输，发育中的脊椎动物胚胎。

The overall objective of this application is to understand the regulation of embryonic calcium metabolism with specific emphasis on the process of transplacental calcium transport. The system chosen for the proposed studies is the chick embryonic chorioallantoic membrane (CAM) which represents the avian equivalent of the placental unit and is responsible for the translocation of eggshell calcium into the embryonic circulation during development. The projects described in this proposal aim to elucidate the biochemical, cellular, and molecular mechanisms regulating the CAM calcium transport function. Our approach is to study the transport-associated proteins (e.g. calcium-binding protein, CaBP; and Ca2+-ATPase) previously identified in this laboratory as functional marker molecules for the calcium transport process. Specifically, we plan to first characterize and localize at a subcellular level these components and analyze their functional involvement and mechanistic role(s) in CAM calcium transport. Secondly, we will study the cellular, biochemical, and molecular events leading to the regulation of CaBP expression during normal embryonic development and under experimentally induced conditions of vitamin K and/or calcium deficiency. We believe that the experiments proposed here will define the functional roles of these marker molecules in CAM calcium transport and provide fundamental information on the mechanisms regulating an important physiological function, transplacental calcium transport, of the developing vertebrate embryo.