CORE--CELL CULTURE, MICROINJECTION/ELECTROPORATION AND HISTOLOGY

核心——细胞培养、显微注射/电穿孔和组织学

• 批准号: 6267678
• 负责人: OLIVIA M. PEREIRA-SMITH
• 金额: $ 20.4万
• 依托单位: BAYLOR COLLEGE OF MEDICINE
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-04-01 至 1999-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6267678
• 关键词: Mycoplasma; antiserum; biomedical facility; electron microscopy; electroporation; histology; immunocytochemistry; in situ hybridization; microinjections; tissue /cell culture; western blottings

Fetal bovine serum is used by all projects and will be tested for optimal growth and cloning efficiency by Core. All the projects will utilize the lot of fetal bovine serum characterized as the best for use, and this will allow for purchase of large amounts of a particular lot for a better price. The Core will also perform regular mycoplasma testing on the various cell cultures used in the individual projects. In addition, Projects 1, 2, 3 and 5 will make use of the microinjection/electroporation facility to introduce macromolecules (primarily RNA, DNA and protein) into cultured mammalian cells. This centralized facility will provide equipment, supplies, and personnel for the whole program. This is a very cost efficient way for all of the investigators to have access to techniques requiring skilled personnel and specialized equipment.

所有项目均使用胎牛血清， 生长和克隆效率。 所有项目将利用 大量的胎牛血清被认为是最好的使用， 将允许购买大量的特定地段，以更好地 价格 中心还将对患者进行定期支原体检测。 在各个项目中使用的各种细胞培养物。 此外，本发明还提供了一种方法， 项目1、2、3和5将利用 微注射/电穿孔设备，以引入大分子 （主要是RNA、DNA和蛋白质）进入培养的哺乳动物细胞。 这 集中设施将提供设备、用品和人员， 整个项目 对于所有人来说，这是一种非常具有成本效益的方式 调查人员能够获得需要熟练人员的技术 和专业设备。