CORE--CELL CULTURE, MICROINJECTION/ELECTROPORATION AND HISTOLOGY

所有项目都使用胎牛血清，并将进行最佳测试 按核心计算的增长和克隆效率。所有项目都将利用 很多胎牛血清被认为是最好的使用，而这 将允许大量购买特定批次以获得更好的 价格。核心还将定期进行支原体测试 在各个项目中使用的各种细胞培养。此外, 项目1、2、3和5将利用 微注入/电穿孔设备引入大分子 (主要是RNA、DNA和蛋白质)进入培养的哺乳动物细胞。这 中央设施将提供设备、用品和人员，以 整个节目。这是一种非常经济高效的方式， 调查人员可以接触到需要熟练人员的技术 和专门的设备。

Fetal bovine serum is used by all projects and will be tested for optimal growth and cloning efficiency by Core. All the projects will utilize the lot of fetal bovine serum characterized as the best for use, and this will allow for purchase of large amounts of a particular lot for a better price. The Core will also perform regular mycoplasma testing on the various cell cultures used in the individual projects. In addition, Projects 1, 2, 3 and 5 will make use of the microinjection/electroporation facility to introduce macromolecules (primarily RNA, DNA and protein) into cultured mammalian cells. This centralized facility will provide equipment, supplies, and personnel for the whole program. This is a very cost efficient way for all of the investigators to have access to techniques requiring skilled personnel and specialized equipment.