Core--Transgenic/targeted mutagenesis

产品说明：转基因小鼠和基因敲除小鼠越来越多地用于癌症研究，以模拟基因扩增和模拟肿瘤抑制基因功能的丧失。此外，这些试剂正在成为高度相关的系统，以测试新的化学预防或化疗药物。 转基因/靶向突变核心的首要目标是处于转化癌症研究的最前沿。 因此，在应用于临床研究之前，转基因和基因敲除小鼠是从细胞培养开始的研究的逻辑和必要的扩展。该核心设施的总体目标是为加州大学弗朗西斯科癌症中心生产转基因创始人和敲除嵌合体小鼠。 此外，核心将提供“模块化”服务，如胚胎再衍生，胚胎冷冻和标准化胚胎干细胞基因靶向实验室与内部专业知识。 此外，核心将致力于优化新兴的鼠遗传操作技术，如嵌合体生产的聚合技术，以及诱导基因表达和组织特异性敲除的标准化转基因系的生产。

Description: (Applicant's Description) Increasingly both transgenic and knockout mice are being used in cancer research to model gene amplification and emulate loss of tumor suppressor gene function. Moreover these reagents are emerging as highly relevant systems to test novel chemopreventive or chemotherapeutic drugs. An overriding goal of the Transgenic/Targeted Mutagenesis Core is to be at the forefront of translational cancer research. As such transgenic and knockout mice are logical and necessary extensions of investigations begun in cell culture, prior to applications to clinical investigations. The overall goals of this Core Facility will be production of transgenic founder and knockout chimera mice for the University of California San Francisco Cancer Center. In addition the Core will provide "modular" services such as embryo rederivation, embryo freezing, and standardized embryonic stem cells for gene targeting for laboratories with in-house expertise. Moreover the Core will be dedicated to optimizing emerging techniques of murine genetic manipulation such as aggregation techniques for chimera production, and production of standardized transgenic lines for both inducible gene expression, and tissue specific knockouts.