METAL COMPLEX CATALYZED HYDROLYSIS OF PHOSPHATE ESTER

该项目的长期目标和具体目标是双重的；a) 为进一步推进我们正在进行的研究项目的综合和详细 单核和多核过渡金属络合物的初步研究 模拟水解酶活性部位的各个方面，并 有助于了解酶的作用机制 催化，以及；b)同样重要的是，招募、参与和培训 医学/生物学专业的少数民族研究生和本科生 相关化学研究作为他们未来培训的一部分 生物医学研究领域的专业人士。 金属水解酶在All的代谢中起着至关重要的作用。 因此有必要了解它们的机制 一般的和特殊的，但总是非常有效的，重要的催化 如生物分子如DNA，RNA， 碳水化合物、脂肪和蛋白质。有人建议合成一系列 合理设计和逐步修饰的配体及其锌(II)， 镍(II)、铜(II)配合物的提纯及其X射线的详细研究 晶体结构、光谱性质及其酸基 不同温度下金属络合物在水溶液中的稳定性平衡 溶剂和pH值的条件。我们会进行详细的研究。 这些络合物的催化性能最初用于小分子的水解性 具有生物相关官能团的分子，如磷酸盐 和羧酸酯和多肽(酰胺)键，以及在以后的阶段 真正的生物分子。催化反应产物将是 确定，研究反应的动力学，并进行调查 探讨了配体和金属络合物特性之间的关系 催化剂的性质以及这些发现如何有助于理解 酵素。 学生参与和科学研究培训：我们的平等 该项目强调的重点和目标是少数民族的培训 本科生和研究生学习技术和方法 与化学/生物和医学相关的化学研究领域 菲尔兹。我们的目标是招收有前途的毕业生和本科生。 霍华德大学和其他学校和学院的学生在 他们学习的早期阶段让学生参与我们正在进行的 每个阶段都有学生积极引导参与的研究工作 从文献研究，实验设计，执行的工作 实验、数据解释和交流研究成果 研讨会和出版物。这是该公司的历史记录 研究小组并将在财政支持下继续进行 获得。

The long term objectives and specific aims of this project are twofold; a) to further our ongoing research project in the synthesis and detailed studies of mono-and multi-nuclear transition metal complexes in an attempt to mimic aspects of the active sites of the hydrolytic enzymes and contribute toward the effort to understand the mechanisms of enzymatic catalysis, and; b) equally importantly, to recruit, involve, and train minority graduate and undergraduate students in the medical/biologically related chemical research as part of their training as prospective professionals in the biomedical field of research. The hydrolytic metallo-enzymes play crucial roles in the metabolism of all living organisms and hence there is a need to understand their mechanism of general and specific but always highly efficient, catalysis of vital reactions such as the hydrolysis of biomolecules such as DNA, RNA, carbohydrates, fats, and proteins. It is proposed to synthesize a range of rationally designed and progressively modified ligands and their Zn(II), Ni(II), and Cu(II) complexes, purify and study in detail their X-ray crystallographic structure, spectroscopic properties, and their acid-based and metal complex stability equilibria in Aqueous solutions under various conditions of solvents and pH. Detailed studies will be carried out of the catalytic properties these complexes initially for the hydrolysis of small molecules with biologically relevant functional groups such as phosphate and carboxylic acid esters and peptide (amide) bonds and at later stages actual biological molecules. Catalytic reaction products will be identified, the kinetics of the reactions studied, and investigations made into the relationship of the ligand and metal complex characteristics to the catalytic properties and how these findings help in understanding the enzymes. Student Participation and training in scientific research: Our equally emphasized focus and aim for this project is the training of minority undergraduate and graduate students in the techniques and methodology in the chemical research field related to chemical/biological and medical fields. We aim to recruit promising graduate and undergraduate minority students within howard university and from other schools and colleges at the early stages of their studies to involve students in our ongoing research work with active guided participation of students at every stage of the work from literature studies, design of experiments, executing experiments, interpreting data and communicating research finding in seminars and in publications. That has been the track record of the research group and it will be continued with the financial support obtained.