PILOT STUDY--CARDIOPROTECTIVE AGENTS FROM TROPICAL FRUITS AND VEGETABLES

试点研究——热带水果和蔬菜中的心脏保护剂

• 批准号: 6254645
• 负责人: EDWARD JAMES KENNELLY
• 金额: $ 6.38万
• 依托单位: HERBERT H. LEHMAN COLLEGE
• 依托单位国家: 美国
• 财政年份: 1985
• 资助国家: 美国
• 起止时间: 1985-06-01 至 2003-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6254645
• 关键词: antioxidants; atherosclerosis; bioassay; biological products; density gradient ultracentrifugation; disease /disorder prevention /control; drug discovery /isolation; drug screening /evaluation; fruit; gas chromatography; heart disorder; high performance liquid chromatography; hot climate; hypercholesterolemia; low density lipoprotein; nuclear magnetic resonance spectroscopy; plant extracts; vegetables

Although phytochemicals have a long history of use in the treatment of diseases, only recently have a number of phytochemical been identified that may prevent certain diseases in humans. The long-term objective of this proposal is to discovery new phytochemicals, especially from dietary sources, that have the potential to prevent coronary heart disease. In this proposal, phytochemicals from tropical fruits and vegetables will be examined for their potential to prevent heart disease. Tropical fruits and vegetables will be used in this study due to their anticipated low toxicity, as well as for the diversity of phyto produced by tropical plant species. Tropical fruits and vegetables will be obtained from US suppliers, as well as from Asian collaborators. Plants will be extracted and tested in an in vitro assay to detect potential cardioprotective agents. The assay described in this proposal is a fast, simple, and reliable test for the inhibition in the scientific literature, and is a method that detects oxidation products using head-space gas chromatography. Extracts that display significant activity in this assay will be subjected to bioactivity- directed isolation. Active phytochemicals will be identified using modern spectroscopic techniques, including one- and two-dimensional nuclear magnetic resonance. Attempts will be made to prevent re-isolation of known inhibitors of LDL-oxidation through literature searches, and by the use of a LC/MS dereplication system. It is expected that this research will lead to the discovery of novel phytochemicals that prevent the oxidation of LDL in vitro. These compounds may also serve as models for potential new therapeutic agents for the treatment of hypercholesterolemia. This is a pilot project for a new investigator.

虽然植物化学物质在治疗疾病方面有着悠久的历史，但直到最近才发现一些植物化学物质可以预防人类的某些疾病。该提案的长期目标是发现新的植物化学物质，特别是从饮食来源，有可能预防冠心病。在这项提案中，将研究热带水果和蔬菜中的植物化学物质对预防心脏病的潜力。本研究将使用热带水果和蔬菜，因为它们预期毒性较低，而且热带植物物种产生的植物多样性也较高。热带水果和蔬菜将从美国供应商以及亚洲合作者那里获得。植物将被提取并在体外试验中进行测试，以检测潜在的心脏保护剂。本提案中描述的测定是科学文献中抑制作用的快速、简单和可靠的检测方法，是一种使用顶空气相色谱法检测氧化产物的方法。在本试验中显示显著活性的浸提液将进行生物活性定向分离。活性植物化学物质将使用现代光谱技术，包括一维和二维核磁共振鉴定。将尝试通过文献检索和使用LC/MS去复制系统来防止已知LDL氧化抑制剂的重新分离。预计这项研究将导致发现新的植物化学物质，防止氧化的LDL在体外。这些化合物也可作为治疗高胆固醇血症的潜在新治疗剂的模型。这是一个新调查员的试点项目。