DNA and Phytochemical Fingerprinting of Actaea Species

Actaea 物种的 DNA 和植物化学指纹图谱

• 批准号: 7268086
• 负责人: EDWARD JAMES KENNELLY
• 金额: $ 16.15万
• 依托单位: HERBERT H. LEHMAN COLLEGE
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-07-01 至 2009-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7268086
• 关键词: Actaea; American; Asia; Asians; Basic Science; Black Cohosh Extract; Botanicals; Chemicals; Cimicifuga racemosa; Classification; Clinical Trials; DNA; DNA Sequence; Data; Development; Diagnostic; European; Fingerprint; Genetic; Genetic Identity; Genetic Polymorphism; Genetic Research; Genetic Variation; Geographic Locations; Goals; Harvest; Head; High Pressure Liquid Chromatography; Hormone replacement therapy; Industry; Institution; Laboratories; Length; Link; Marketing; Measures; Menopause; Methods; New York City; North America; Phytochemical; Plant Roots; Plants; Population; Preparation; Quality Control; Range; Research; Rhizome; Seasonal Variations; Structure; Techniques; Universities; Variant; Woman; base; college; dietary supplements; genetic profiling; improved; interest; liquid chromatography mass spectrometry; programs

DESCRIPTION (provided by applicant): Black cohosh (Actaea racemosa, syn. Cimicifuga racemosa) is a popular dietary supplement and currently one of the most promising alternative approaches to hormone replacement therapy for menopausal women. The root and rhizomes, often collected by wild-crafting, are typically used for botanical preparations. There are several species of Actaea that co-occur with black cohosh in the US, and some of these are difficult to differentiate. In addition, Asian Actaea species are being sold as black cohosh. Good botanical and manufacturing practices are hampered by the lack of adequate data about the botanical identification and phytochemistry of the different species in this genus. This study involves the examination of variation in Actaea at the species, population, and seasonal levels. We plan to use DNA and phytochemical fingerprinting methods to distinguish A. racemosa from closely related Actaea species by examining variation in eight North American and four Asian species by DNA barcoding, high-performance liquid chromatography-photodiode array (HPLC-PDA), and liquid chromatography-mass spectrometry (LC-MS) methods that we have developed in our laboratories. We will fingerprint the distinguishing features of 20 populations of black cohosh using phytochemical and DNA (amplified fragment length polymorphisms) methods. We will also examine the seasonal variation of black cohosh constituents. The data will be used to improve the identification of black cohosh. For both basic research on plant systematics and the safe use of black cohosh, it is important to develop reliable DNA and phytochemical fingerprinting methods to identify Actaea species. Our long-range goals are to improve the quality of black cohosh dietary supplements and understand the variation in Actaea species.

描述(申请人提供)：黑升麻(总状花序Actaea racemosa，sy.Cimicifuga racemosa)是一种流行的膳食补充剂，目前是更年期妇女激素替代疗法最有前途的替代方法之一。根和根茎，通常通过野生手工采集，通常用于植物制剂。在美国，有几种Actaea与黑升麻共生，其中一些很难区分。此外，亚洲升麻树种正被当作黑升麻出售。良好的植物学和生产实践由于缺乏关于该属不同物种的植物学鉴定和植物化学的充分数据而受到阻碍。这项研究涉及在物种、种群和季节水平上对Actaea的变异进行检查。我们计划使用DNA和植物化学指纹图谱方法，通过我们实验室开发的DNA条形码、高效液相-光电二极管阵列(HPLC-PDA)和液-质联用(LC-MS)方法检测8个北美和4个亚洲物种的变异，以区分总状花序和近缘Actaea物种。我们将使用植物化学和DNA(扩增片段长度多态)方法对20个黑升麻种群的特征进行指纹图谱分析。我们还将研究黑升麻成分的季节变化。这些数据将用于改进黑升麻的鉴定。为了植物系统学的基础研究和黑升麻的安全使用，建立可靠的DNA和植物化学指纹图谱方法对猕猴桃属植物进行鉴定具有重要意义。我们的长期目标是提高黑升麻膳食补充剂的质量，并了解Actaea物种的变异。