PILOT STUDY--CARDIOPROTECTIVE AGENTS FROM TROPICAL FRUITS AND VEGETABLES

虽然植物化学物质在疾病治疗中的使用历史很长，但直到最近才发现一些植物化学物质可以预防人类的某些疾病。这项提议的长期目标是发现新的植物化学物质，特别是来自饮食来源的化学物质，这些化学物质有可能预防冠心病。在这项提案中，来自热带水果和蔬菜的植物化学物质将被检查其预防心脏病的潜力。热带水果和蔬菜将被用于这项研究，因为它们预期毒性低，以及热带植物物种产生的植物的多样性。热带水果和蔬菜将从美国供应商以及亚洲合作者那里获得。植物将被提取并在体外试验中进行测试，以检测潜在的心脏保护剂。本建议中描述的方法是科学文献中关于抑制作用的快速、简单和可靠的测试，并且是一种使用顶空气相色谱检测氧化产物的方法。在这项检测中显示显著活性的提取物将受到生物活性指导的分离。活性植物化学物质将使用现代光谱技术进行鉴定，包括一维和二维核磁共振。将尝试通过文献搜索和使用LC/MS去复制系统来防止已知的低密度脂蛋白氧化抑制物的重新分离。预计这项研究将导致发现在体外防止低密度脂蛋白氧化的新植物化学物质。这些化合物还可以作为治疗高胆固醇血症的潜在新治疗药物的模型。这是一个新调查员的试点项目。

Although phytochemicals have a long history of use in the treatment of diseases, only recently have a number of phytochemical been identified that may prevent certain diseases in humans. The long-term objective of this proposal is to discovery new phytochemicals, especially from dietary sources, that have the potential to prevent coronary heart disease. In this proposal, phytochemicals from tropical fruits and vegetables will be examined for their potential to prevent heart disease. Tropical fruits and vegetables will be used in this study due to their anticipated low toxicity, as well as for the diversity of phyto produced by tropical plant species. Tropical fruits and vegetables will be obtained from US suppliers, as well as from Asian collaborators. Plants will be extracted and tested in an in vitro assay to detect potential cardioprotective agents. The assay described in this proposal is a fast, simple, and reliable test for the inhibition in the scientific literature, and is a method that detects oxidation products using head-space gas chromatography. Extracts that display significant activity in this assay will be subjected to bioactivity- directed isolation. Active phytochemicals will be identified using modern spectroscopic techniques, including one- and two-dimensional nuclear magnetic resonance. Attempts will be made to prevent re-isolation of known inhibitors of LDL-oxidation through literature searches, and by the use of a LC/MS dereplication system. It is expected that this research will lead to the discovery of novel phytochemicals that prevent the oxidation of LDL in vitro. These compounds may also serve as models for potential new therapeutic agents for the treatment of hypercholesterolemia. This is a pilot project for a new investigator.