CORE--MUTANT RODENT RESOURCE

核心——突变啮齿动物资源

• 批准号: 6299912
• 负责人: Norman R. Drinkwater
• 金额: $ 21.64万
• 依托单位: UNIVERSITY OF WISCONSIN-MADISON
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-04-01 至 2001-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6299912
• 关键词: biomedical facility; cryopreservation; fusion gene; genetically modified animals; laboratory mouse; laboratory rat

Research over the preceding grant period has focused increasingly on the use of transgenic and mutant animals for our studies of neoplastic development. The Mutant Rodent Resource, a new shared service, will provide McArdle scientists with the capacity to manipulate the genome of experimental animals by providing ready access to the following technologies: . Generation of transgenic mice and rats by pronuclear microinjection . Generation of chimeric mice by microinjection of embryonic stem cells into host blastocysts . Cryopreservation of mouse sperm

在前一个赠款期的研究越来越集中在 转基因和突变动物在肿瘤研究中的应用 发展变种啮齿动物资源，一个新的共享服务，将 为麦卡德尔科学家提供了操纵基因组的能力， 通过提供以下便利， 技术： .原核显微注射法制备转基因小鼠和大鼠 .显微注射胚胎干细胞构建嵌合体小鼠 进入宿主胚泡 .小鼠精子的冷冻保存