CORE--TRANSGENIC ANIMAL

核心——转基因动物

• 批准号: 6299987
• 负责人: Carl A. Pinkert
• 金额: $ 19.59万
• 依托单位: UNIVERSITY OF ALABAMA AT BIRMINGHAM
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-03-03 至 2001-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6299987
• 关键词: animal breeding; animal care; animal colony; biomedical facility; biomedical resource; cryopreservation; disease /disorder model; gene targeting; genetic manipulation; genetically modified animals; germ free condition; laboratory mouse; model design /development; veterinary medicine

The Transgenic Animal/Embryonic Stem Cell Shared Facility was initiated in 1991 and became a Cancer Center shared facility in 1995. Its purpose is to provide Cancer Center investigators with pathogen-free transgenic mice with high standards of quality control and efficiency. The service also provides advice and consultation to first-time users, re- derivation of stocks, cryopreservation and associated technologies. Since inception, it has injected 500 genes and 40 ES cell clones including "knock-out" and "knock-in" models. Over 3,5000 founder animals have been provided. Over the past two years, the facility has provided 248 effort days per year with 23 different funded Cancer Center members from five program areas. Increasing volume and sources of revenue has allowed a reduction in charges to investigators from $1,500/project to $750/project in 199. Further expansion of utilization is projected based on institutional expansion of genetics facilities and faculty members.

转基因动物/胚胎干细胞共享设施于1991年启动，并于1995年成为癌症中心共享设施。其目的是为癌症中心研究人员提供具有质量控制和效率高标准的无病原体转基因小鼠。该服务还为首次用户，股票的推导，冷冻保存和相关技术提供建议和咨询。自成立以来，它已经注入了500个基因和40个ES细胞克隆，包括“敲除”和“敲门”模型。已经提供了超过3,5000种创始人动物。在过去的两年中，该设施每年提供248个努力日，来自五个计划领域的23个不同资金的癌症中心成员。收入的数量和收入来源的增加使调查人员的收费从1,500美元/项目减少到199年的750美元。基于遗传学设施和教职员工的机构扩展，预计利用率的进一步扩大。