CORE--ORGANIC SYNTHESIS FACILITY

核心——有机合成装置

• 批准号: 6300127
• 负责人: SHANTU G AMIN
• 金额: $ 26.2万
• 依托单位: INSTITUTE FOR CANCER PREVENTION
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-12-22 至 2000-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6300127
• 关键词: biomedical facility; carcinogens; chemical synthesis; organic chemicals

The Organic Synthesis Facility supports the Cancer Center's research activities in environmental carcinogenesis, and chemoprevention, and the identification of host factors of etiologic significance by providing chemically pure compounds for model assays, as reference materials, and as internal standards. It is a dedicated resource occupying 900 sq. ft. in Unit 1 and 447 sq. ft on the lower level of the NDI in Valhalla. The CCSG- supported staff are the Facility Head, Shantu Amin, Ph.D. and one organic chemist, Jacek Krzeminski, Ph.D. The Facility supports the Cancer Center's research activities in environmental carcinogenesis, and chemoprevention, and the identification of host factors of etiologic significance by providing chemically pure compounds for model assays, as reference materials, and as internal standards. Requested compounds are furnished to AHFCC's investigators in compounds is ensured by state-of-the-art technology, including HPLC, GC-MS, TLC, UV, IR, FTIR, MS, and NMR. The scientists in the facility are highly experienced in developing efficient synthetic routes for cold and radioisotope-labeled polynuclear aromatic hydrocarbons, tobacco-specific nitrosamines, and other environmental and nutritional carcinogens as well as their metabolites, and their DNA and protein adducts. The Facility's dedicated Carcinogen Synthesis Laboratory is equipped with high pressure liquid chromatographs including one instrument with a beta- flo-one model IC radioactivity detector, and with a medium pressure liquid chromatograph. These instruments enable the analysis and purification of starting materials, intermediates, and end products. The laboratory has hoods with glove box, a walk-in hood as required by regulatory statutes, and a special photolysis set-up. The dedicated selenium laboratory, in which chemoprevention agents are prepared, has a special exhaust system in compliance with safety regulations pertaining to these chemicals. The dedicated Synthesis Laboratory for intermediates, nucleoside markers, and chemopreventive agents is equipped with a photolysis set-up. HPLC with photo diode array detector, and two working hoods with a 24-hour exhaust system. Radiolabeled syntheses are carried out in another dedicated laboratory that is accessed only upon approval and is also supervised by the AHFCC's Safety Officer.

有机合成设施支持癌症中心的研究 环境致癌和化学预防的活动，以及 用提供的方法鉴定具有病因学意义的宿主因素 用于模型分析、作为参考物质和AS的化学纯化合物 内部标准。它是一个占地900平方米的专用资源。英国《金融时报》在……里面 1单元和447平方英尺英国《金融时报》对瓦尔哈拉NDI较低级别的报道。CCSG- 支持的工作人员是设施负责人Shantu Amin博士和一名有机人员 化学家Jacek Krzinski博士。该设施支持癌症中心的 环境致癌和化学预防方面的研究活动， 以及病因学上有意义的宿主因素的鉴定 提供用于模型分析的化学纯化合物作为参考 材料，并作为内标。要求的化合物被提供给 AHFCC在化合物方面的调查人员得到了最先进的保证 技术包括高效液相色谱、GC-MS、TLC、UV、IR、FTIR、MS和核磁共振。这个 该设施中的科学家在开发高效的 冷和放射性同位素标记多核芳烃的合成路线 碳氢化合物、烟草特有的亚硝胺和其他环境和 营养致癌物及其代谢物，以及它们的DNA和 蛋白质加合物。 该设施的专门致癌物合成实验室配备了 高压液相色谱仪包括一个带有贝塔的仪器- Flo-One IC型放射性探测器，配有中压液体 色谱仪。这些仪器使分析和提纯 原料、中间体和最终产品。实验室里有 带有手套箱的引擎盖，监管法规要求的步入式引擎盖， 和一种特殊的光解装置。专门的硒实验室，在 哪种化学防腐剂是配制的，有一个特殊的排气系统 遵守与这些化学品有关的安全法规。这个 中间体、核苷标记物和 化学防腐剂配备了光解装置。高效液相色谱仪 光电二极管阵列探测器，以及两个24小时排气的工作罩 系统。放射性标记的合成是在另一个专门的 只有在获得批准后才能进入的实验室，也由 AHFCC的安全官员。