CORE--ORGANIC SYNTHESIS FACILITY

核心——有机合成装置

• 批准号: 6413601
• 负责人: SHANTU G AMIN
• 金额: $ 24.29万
• 依托单位: INSTITUTE FOR CANCER PREVENTION
• 依托单位国家: 美国
• 财政年份: 2001
• 资助国家: 美国
• 起止时间: 2001-01-26 至 2001-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6413601
• 关键词: biomedical facility; carcinogens; chemical synthesis; organic chemicals

The Organic Synthesis Facility supports the Cancer Center's research activities in environmental carcinogenesis, and chemoprevention, and the identification of host factors of etiologic significance by providing chemically pure compounds for model assays, as reference materials, and as internal standards. It is a dedicated resource occupying 900 sq. ft. in Unit 1 and 447 sq. ft on the lower level of the NDI in Valhalla. The CCSG- supported staff are the Facility Head, Shantu Amin, Ph.D. and one organic chemist, Jacek Krzeminski, Ph.D. The Facility supports the Cancer Center's research activities in environmental carcinogenesis, and chemoprevention, and the identification of host factors of etiologic significance by providing chemically pure compounds for model assays, as reference materials, and as internal standards. Requested compounds are furnished to AHFCC's investigators in compounds is ensured by state-of-the-art technology, including HPLC, GC-MS, TLC, UV, IR, FTIR, MS, and NMR. The scientists in the facility are highly experienced in developing efficient synthetic routes for cold and radioisotope-labeled polynuclear aromatic hydrocarbons, tobacco-specific nitrosamines, and other environmental and nutritional carcinogens as well as their metabolites, and their DNA and protein adducts. The Facility's dedicated Carcinogen Synthesis Laboratory is equipped with high pressure liquid chromatographs including one instrument with a beta- flo-one model IC radioactivity detector, and with a medium pressure liquid chromatograph. These instruments enable the analysis and purification of starting materials, intermediates, and end products. The laboratory has hoods with glove box, a walk-in hood as required by regulatory statutes, and a special photolysis set-up. The dedicated selenium laboratory, in which chemoprevention agents are prepared, has a special exhaust system in compliance with safety regulations pertaining to these chemicals. The dedicated Synthesis Laboratory for intermediates, nucleoside markers, and chemopreventive agents is equipped with a photolysis set-up. HPLC with photo diode array detector, and two working hoods with a 24-hour exhaust system. Radiolabeled syntheses are carried out in another dedicated laboratory that is accessed only upon approval and is also supervised by the AHFCC's Safety Officer.

有机合成设施支持癌症中心的研究 环境致癌作用和化学预防，以及 通过提供病原学意义的宿主因素的鉴定 用于模型测定的化学纯化合物，作为参考材料，以及作为 内部标准。这是一个专门的资源占用900平方米。英尺在 单位1和447平方米。在瓦尔哈拉的NDI下层。CCSG- 支持的工作人员是设施负责人Shantu Amin博士。和一个有机 化学家Jacek Krzeminski博士该机构支持癌症中心的 环境致癌作用和化学预防方面的研究活动， 并通过以下方法鉴定具有病原学意义的宿主因素： 提供用于模型测定的化学纯化合物，作为参考 材料，并作为内部标准。所需化合物提供给 AHFCC的化合物研究人员由最先进的 技术，包括HPLC、GC-MS、TLC、UV、IR、FTIR、MS和NMR。的 该设施的科学家在开发高效的 冷同位素标记多核芳香化合物合成路线 碳氢化合物，烟草特有的亚硝胺，以及其他环境和 营养致癌物及其代谢物，以及它们的DNA， 蛋白质加合物。 该设施的专用致癌物合成实验室配备有 高压液相色谱仪，包括一台带有β- flo-one型IC放射性检测器，并配有中压液体 色谱仪。这些仪器能够分析和纯化 起始材料、中间体和最终产品。实验室已 带手套箱的通风柜，法规要求的步入式通风柜， 和特殊的光解装置专门的硒实验室，在 其具有特殊的排气系统， 遵守与这些化学品有关的安全法规。的 专用合成实验室，用于中间体、核苷标记物和 化学预防剂配备有光解装置。HPLC以 光电二极管阵列检测器，以及两个24小时排气的工作罩 系统放射性标记的合成在另一个专用的 只有在获得批准后才能进入的实验室，并由 AHFCC的安全官员。