MONITORING A SIDS MODEL IN NEONATAL SWINE

监测新生猪 SIDS 模型

• 批准号: 6387591
• 负责人: Anthony Louis Sica
• 金额: $ 32.11万
• 依托单位: SUNY DOWNSTATE MEDICAL CENTER
• 依托单位国家: 美国
• 财政年份: 1991
• 资助国家: 美国
• 起止时间: 1991-09-30 至 2003-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6387591
• 关键词: afferent nerve; baroreceptors; blood chemistry; denervation; disease /disorder etiology; disease /disorder model; electrocardiography; electroencephalography; heart electrical activity; heart function; heart innervation; heart rhythm; hypercapnia; hypoxia; infant animal; model design /development; sleep; statistics /biometry; sudden infant death syndrome; swine; ventricular fibrillation; videotape /videodisc

The long-term objective of the proposed research is to evaluate the role of cardiac mechanisms in the etiology of sudden death in the Sudden Infant Death Syndrome (SIDS). Thus this project is directly related to a problem in infant survival and health. The specific aims of the proposed research are designed to test the hypothesis that a developmental anomaly in cardiac innervation could reduce ventricular electrical stability and favor the onset of sudden cardiac death. The research design and surgical methods have been worked out in the previous grant period; newborn swine with surgically induced imbalance in cardiac autonomic innervation will be continuously monitored (24 hrs.) throughout eight postnatal weeks. Those surviving and similarly surgically prepared littermates will be tested (at ages 2,4,6,8 wks) for cardiovascular responses to: (i) hypoxia, (ii) hypercapnia, (iii) stimulation of cardiopulmonary receptors, and (iv) alterations in baroreceptor afferent inputs. Results obtained from study of these possible precipitating factors should reveal the increased susceptibility of the neonate at risk for sudden death. Sleep is the predominant behavioral state of the neonate and ontogeny of EEG sleep in neonates can be monitored by serial EEG recordings. We plan to examine ECG recordings as a function of state determined by VCR tapes of behavior, diaphragmatic EMG and EEG, and correlate these variables against both age and type of denervation. Nonlinear analysis of heart rate variability in infants has revealed an increase in complexity with maturation and our own results included similar findings in piglets. This grant proposal would pursue these findings and the effects of selected cardiac denervation on this complexity, correlating results with sudden death in denervated piglets. Such studies should confirm our working hypothesis regarding the importance of cardiac innervation in the etiology of sudden death in infants. We are, therefore, postulating an abnormality at the effector level, i.e., at the cardiac level, specifically: maturation of the normal innervation of the heart and its reflex control.

拟议研究的长期目标是评估 心脏机制在猝死病因学中的作用 婴儿死亡综合症（SIDS）。 因此，该项目直接关系到 婴儿存活和健康的问题。 该委员会的具体目标 拟议的研究旨在验证假设， 心脏神经支配的发育异常可以减少心室肌的收缩， 电稳定性，并有利于心脏性猝死的发生。 的 研究设计和手术方法已经制定出来， 上一个资助期;手术诱导失衡的新生猪 将连续监测心脏自主神经支配（24 小时）在产后的八周内。 那些幸存下来的人， 将对经手术准备的同窝仔进行以下测试（在2、4、6、8周龄时）： 心血管反应：（i）缺氧，（ii）高碳酸血症，（iii） 心肺受体的刺激，以及（iv） 压力感受器传入输入 从这些研究中获得的结果 可能的诱发因素应该揭示出 有猝死风险的新生儿的易感性。 睡眠是 新生儿的主导行为状态与EEG睡眠的个体发生 可以通过连续的EEG记录来监测。 我们计划 检查ECG记录作为VCR磁带确定的状态的函数 的行为，脑肌电图和脑电图，并将这些变量 与年龄和去神经类型的对比。 心脏的非线性分析 婴儿的心率变异性揭示了复杂性的增加， 成熟和我们自己的结果包括在小猪中的类似发现。 这项赠款提案将继续研究这些发现和 选择心脏去神经支配的复杂性，相关结果 去神经小猪的猝死 这些研究应该证实 我们关于心脏神经支配重要性的假设 婴儿猝死的病因学研究 因此， 假设效应物水平的异常，即，在心脏 水平，特别是：心脏正常神经支配的成熟 和它的反射控制。