CARNITINE PALMITOYL TRANSFERASE & FATTY ACID METABOLISM

肉碱棕榈酰转移酶

• 批准号: 6335257
• 负责人: DENIS MCGARRY
• 金额: $ 1.39万
• 依托单位: UT SOUTHWESTERN MEDICAL CENTER
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-08-15 至 2001-08-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6335257
• 关键词: Mammalia; biomedical resource; musculoskeletal system; nervous system; nuclear magnetic resonance spectroscopy; technology /technique

A central question in exercise physiology is how the balance between carbohydrate and fatty acid oxidation in skeletal muscle is altered to meet the metabolic demands of muscle contraction. We're using 13C NMR and non-steady-state isotopomer analysis to directly quantify the relative oxidation of two competing substrates in rat skeletal muscle. We found that [13C]acetate and [13C]lactate were taken up and oxidized by both resting and contracting skeletal muscle, and that high-intensity muscle contractions altered the pattern of substrate utilization such that the relative oxidation of acetate decreased while that of lactate remained unchanged or increased. However, because of the low sensitivity of 13C NMR, we had to pool two gastrocnemius muscles and four soleus muscles in order to acquire interpretable 13C NMR spectra. To overcome this limitation, we recently developed and validated a more sensitive method to analyze 13C enrichment in tissue extracts using 1H NMR. Using this new method, we have obtained data from individual rat gastrocnemius (~1.5 g wet weight) and soleus (~0.15 g wet weight) muscles. Glutamate C4 enrichment values obtained by 1H NMR were highly correlated with measurements obtained by 13C NMR non-steady-state isotopomer analysis. We are now applying these same techniques to examine muscle metabolism in mice. Our preliminary results indicate that 1H NMR is sensitive enough to detect glutamate C4 fractional enrichment in extracts from tissue samples as small as the mouse soleus muscle (10 mg wet weight). (Collaborative 5) REPORT PERIOD: (09/01/97-08/31/98)

运动生理学的一个核心问题是如何平衡 骨骼肌中的碳水化合物和脂肪酸氧化之间 改变以满足肌肉收缩的代谢需求。 是 使用13C NMR和非稳态同位素分析直接 量化大鼠两种竞争底物的相对氧化 骨骼肌。 我们发现[13C]乙酸盐和[13c]乳酸是 被静止和收缩的骨骼肌吸收和氧化 高强度的肌肉收缩改变了 底物利用率使乙酸的相对氧化 乳酸的降低保持不变或增加。 但是，由于13c NMR的灵敏度低，我们不得不池两个 为了获得 可解释的13C NMR光谱。 为了克服这一限制，我们 最近开发并验证了一种更敏感的方法来分析 使用1H NMR在组织提取物中富集13C。 使用这个新 方法，我们从单个大鼠胃g的数据中获得了数据（〜1.5 g湿重）和比目鱼（〜0.15克湿重）肌肉。 谷氨酸C4 通过1H NMR获得的富集值与 通过13C NMR非稳态同位素分析获得的测量。 我们现在正在应用这些相同的技术来检查肌肉代谢 在老鼠中。 我们的初步结果表明1H NMR是敏感的 足以检测到提​​取物中提取物的谷氨酸C4分数富集 组织样品与小鼠比目鱼肌（10毫克湿重）一样小。 （协作5）报告期：（09/01/97-08/31/98）