NMR STUDIES OF ISOTOPICALLY ENRICHED HUMAN INTERLEUKIN 5
NMR STUDIES OF ISOTOPICALLY ENRICHED HUMAN INTERLEUKIN 5
批准号:
6309128
负责人:
RONALD D GUILES
金额:
$0.75万
依托单位国家:
美国
项目类别:
财政年份:
2000
资助国家:
美国
项目状态:
已结题
起止时间:
2000-04-15 至 2005-02-28
中文摘要
我们已经开始了一项旨在获得解决方案的研究
英文摘要
We have initiated a study directed at obtaining the solution
structure of human interleukin-5. Interleukin-5 is a homodimeric
protein with a molecular weight of 25 kDaltons. It is unique among
the cytokines involved in hematopoesis in that it is essential in
eosinsophil differentiation. It is induced upon presentation of
foreign bodies or parasites. As such it has become a key target for
drug design efforts in the alleviation of asthma, a condition which is
often associated with eosinophilia and the inflammatory response.
Although a crystal structure is known, we believe determining a
complete set of assignments and a solution structure will facilitate
the study of small molecule interactions with the protein in regions
that site-directed mutagenesis experiments indicate are essential in
receptor interaction. Our efforts initially focused on obtaining a
high yield expression system for the protein using a synthetic gene
approach utilizing optimized codon usage and a high yield expression
vector. Using this system we have obtained sufficient quantities of
doubly labeled interleukin-5 to begin triple resonance assignment and
structure elucidation.
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NOVEL NMR STUDIES OF HEME PROTEINS INVOLVED IN ANEMIA
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批准号:6309127
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项目类别:
-
资助金额:$0.75万
-
财政年份:2000
-
负责人:RONALD D GUILES
-
依托单位:
NOVEL NMR STUDIES OF HEME PROTEINS INVOLVED IN ANEMIA
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批准号:6298124
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项目类别:
-
资助金额:$0.75万
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财政年份:1999
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负责人:RONALD D GUILES
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依托单位:
NMR STUDIES OF ISOTOPICALLY ENRICHED HUMAN INTERLEUKIN 5
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批准号:6298125
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项目类别:
-
资助金额:$0.75万
-
财政年份:1999
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负责人:RONALD D GUILES
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依托单位:
NOVEL NMR STUDIES OF HEME PROTEINS INVOLVED IN ANEMIA
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批准号:6281563
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项目类别:
-
资助金额:$0.15万
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财政年份:1998
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负责人:RONALD D GUILES
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依托单位:
NMR STUDIES OF ISOTOPICALLY ENRICHED HUMAN INTERLEUKIN 5
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批准号:6281564
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项目类别:
-
资助金额:$0.79万
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财政年份:1998
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负责人:RONALD D GUILES
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依托单位:
NMR STUDIES OF ISOTOPICALLY ENRICHED HUMAN INTERLEUKIN 5
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批准号:6252078
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项目类别:
-
资助金额:$0.52万
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财政年份:1997
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负责人:RONALD D GUILES
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依托单位:
TRAINING IN USE OF DMX ELECTRONICS
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批准号:6252079
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项目类别:
-
资助金额:$0.52万
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财政年份:1997
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负责人:RONALD D GUILES
-
依托单位:
NOVEL NMR STUDIES OF HEME PROTEINS INVOLVED IN ANEMIA
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批准号:2145739
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项目类别:
-
资助金额:$7.77万
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财政年份:1993
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负责人:RONALD D GUILES
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依托单位:
NOVEL NMR STUDIES OF HEME PROTEINS INVOLVED IN ANEMIA
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批准号:2145738
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项目类别:
-
资助金额:$7.35万
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财政年份:1993
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负责人:RONALD D GUILES
-
依托单位:
NOVEL NMR STUDIES OF HEME PROTEINS INVOLVED IN ANEMIA
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批准号:3464886
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项目类别:
-
资助金额:$10.61万
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财政年份:1993
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负责人:RONALD D GUILES
-
依托单位:
NOVEL NMR STUDIES OF HEME PROTEINS INVOLVED IN ANEMIA
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批准号:2145737
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项目类别:
-
资助金额:$13.64万
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财政年份:1993
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负责人:RONALD D GUILES
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依托单位:
NOVEL NMR STUDIES OF HEME PROTEINS INVOLVED IN ANEMIA
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批准号:2430208
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项目类别:
-
资助金额:$8.22万
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财政年份:1993
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负责人:RONALD D GUILES
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依托单位:
海外基金