IMMUNOASSAYS FOR ALPHAFETOPROTEIN: FETAL DVMT & TUMOR GROWTH

α胎蛋白的免疫测定：胎儿 DVMT

• 批准号: 6328005
• 负责人: JOHN P NOLAN
• 金额: $ 0.77万
• 依托单位: UNIVERSITY OF CALIF-LOS ALAMOS NAT LAB
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-07-01 至 2001-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6328005
• 关键词: biomedical resource; genetics; technology /technique

Alphafetoprotein (AFP) is a fetal glycoprotein that is associated with the normal growth of the human fetus as with cancerous tumors. For more than three decades, AFP has been used as both a tumor marker and as a birth defect screening agent. More recently, Mizejewski and colleagues have identified a form of AFP in which a normally hidden, growth inhibitory site (GIS) is exposed. This partially denatured, molten glubule form of AFP is likely to play an important role in regulating fetal development and tumor growth. An assay that could rapidly and quantitatively measure this altered form of AFP could be a valuable diagnostic tool. The goal of this project is to develop a sensitive and quantitative assay for the two forms of AFT using microsphere-based flow cytometry.

甲胎蛋白(AFP)是一种胎儿糖蛋白，与 人类胎儿的正常生长，就像癌症一样。 三十多年来，甲胎蛋白一直被用作肿瘤标记物 并作为出生缺陷筛查剂。最近，米泽耶夫斯基和 同事们已经确定了一种形式的AFP，在这种形式中，通常隐藏着一种 暴露出生长抑制部位(GIS)。这部分变性了， 熔融的谷蛋白形式的甲胎蛋白可能在 调节胎儿发育和肿瘤生长。一种可以 快速和定量检测这种改变形式的甲胎蛋白可能是 有价值的诊断工具。这个项目的目标是开发一种 两种AFT的灵敏定量检测 基于微球的流式细胞术。