ANTIMITOTIC DRUGS & MICROTUBULE MEDIATED SIGNAL TRANSDUCTION

抗有丝分裂药物

• 批准号: 6308877
• 负责人: THOMAS WANDLESS
• 金额: $ 0.99万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN FRANCISCO
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-03-01 至 2002-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6308877
• 关键词: biological products; biomedical resource; spectrometry; structural biology

There is evidence indicating that antigenic peptides are bound to chaperonin proteins and transported to the MHC class I by these chaperonins. More experimental results are needed to establish the detailed mechanism. We will use mass spectrometry based techniques to analyze the chaperonin protein GRP 94/peptides complex and peptides isolated from GRP 94 to address this challenge. The objectives of this research project are: 1. Obtain first-hand evidence that antigenic peptides are complexed with GRP 94. 2. Determine the sequence of these antigenic peptides. 3. Determine the sites where antigenic peptides are bound. Preliminary results have shown the success of mass spectrometry in sequencing peptides isolated from GRP 94 by HPLC. One peptide has been sequenced by MALDI/PSD MS. Further work will hopefully fulfill our goal.

有证据表明，抗原肽结合